The proposition of a mutant of Lep that would test for the "inside positive" rule of the orientation of proteins in the membranes. Concept introduction: Positive inside rule: From photosynthetic RC, the statistical analysis revealed in E.Coli and protein a prevalence of basic residues in the cytoplasmic loops it can e determined by using topological data. This rule helps to predict the orientation of the TM helices from a sequence.

Question

Want to see the full answer?

Answer 1

Question

Chapter 10, Problem 18P

Interpretation Introduction

Interpretation:

The proposition of a mutant of Lep that would test for the "inside positive" rule of the orientation of proteins in the membranes.

Concept introduction:

Positive inside rule:

From photosynthetic RC, the statistical analysis revealed in E.Coli and protein a prevalence of basic residues in the cytoplasmic loops it can e determined by using topological data.

This rule helps to predict the orientation of the TM helices from a sequence.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin, chymotrypsin, and cyanogen bromide treatments are shown: ⚫ Trypsin digestion gave two fragments with multiple residues (not in order): • T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr o T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe • CT2: Thr, Trp • CT3: Lys, Met, Tyr, Val 。 CT4: Arg, Gly ⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between each residue.

A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin, chymotrypsin, and cyanogen bromide treatments are shown: • Trypsin digestion gave two fragments with multiple residues (not in order): T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr • T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CTI: Ala, Phe 。 CT2: Thr, Trp • CT3: Lys, Met, Tyr, Val 。 CT4: Arg, Gly ⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between each residue.

Poly-L-leucine in an organic solvent such as dioxane is a helical, whereas poly-L-isoleucine is not. Why do these amino acids with the same number and kinds of atoms have different helix-forming tendencies? L-isoleucine's methyl group sterically interferes with α-helix formation, whereas L-leucine's methyl group does not interfere. L-isoleucine lacks an NH group that can donate a hydrogen bond to stabilize an a helix, whereas L-leucine has an NH group. L-isoleucine contains a hydrogen-bond acceptor in close proximity to the main chain, whereas L-leucine lacks this group. L-isoleucine's ring structure prevents it from assuming the op value to fit into an a helix, whereas L-isoleucine does not have a ring structure.