The reason for formation of biofilms by some bacteria after being exposed to antibiotics. Introduction : Biofilms are the colonies formed by different species of bacteria. These colonies are initiated by one bacterium that attaches to the surface of vulnerable body parts, like tooth or lung tissue. The other microbes subsequently attach to either the first bacterium or the inevitable sugar and protein secretions by it. Biofilms are present in almost every natural environment, and it is an adaptation strategy of bacteria for the sustenance of their lives. The different species of bacteria cooperate and interact with each other while forming biofilms, and functioning in these colonies.

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Chapter 10, Problem 15Q

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To describe:

The reason for formation of biofilms by some bacteria after being exposed to antibiotics.

Introduction:

Biofilms are the colonies formed by different species of bacteria. These colonies are initiated by one bacterium that attaches to the surface of vulnerable body parts, like tooth or lung tissue. The other microbes subsequently attach to either the first bacterium or the inevitable sugar and protein secretions by it.

Biofilms are present in almost every natural environment, and it is an adaptation strategy of bacteria for the sustenance of their lives. The different species of bacteria cooperate and interact with each other while forming biofilms, and functioning in these colonies.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…