Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Chapter 10, Problem 20Q
Summary Introduction
To describe:
If the treatment of a patient exhibiting signs and symptoms of severe septic shock be done immediately with broad-spectrum or narrow-spectrum drugs.
Introduction:
Broad-spectrum antimicrobials are the drugs that can act against more than one group of bacteria. They don’t require blood test to be prescribed so they are prescribed very often, due to which the bacteria become resistant to them. They also lead to disruption to microbiome. On contrary, narrow spectrum antimicrobials are the drugs that act against only a specific group of bacteria. They require thorough blood test to be prescribed and don’t cause disruption to microbiome.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 10 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 10.1 - State the main goal of antimicrobial treatment.Ch. 10.1 - Identify the sources for the most commonly used...Ch. 10.1 - Describe two methods for testing antimicrobial...Ch. 10.1 - Prob. 4AYPCh. 10.1 - NCLEX PREP 1. An RN is caring for a 26-year-old...Ch. 10.2 - Explain the concept of selective toxicity.Ch. 10.2 - List the five major targets of antimicrobial...Ch. 10.2 - Prob. 7AYPCh. 10.2 - Distinguish between broad-spectrum and...Ch. 10.2 - Prob. 9AYP
Ch. 10.2 - Explain the mode of action of penicillinases and...Ch. 10.2 - Identify two antimicrobials that act by inhibiting...Ch. 10.2 - Prob. 12AYPCh. 10.2 - Identify one example of a fluoroquinolone.Ch. 10.2 - Describe the mode of action of drugs that target...Ch. 10.2 - Prob. 15AYPCh. 10.2 - Prob. 16AYPCh. 10.2 - Explain why antiprotozoal and antihelminthic drugs...Ch. 10.2 - List the three major targets of action of...Ch. 10.2 - Prob. 2NPCh. 10.3 - Discuss two main ways that microbes acquire...Ch. 10.3 - List five cellular or structural mechanisms that...Ch. 10.3 - Prob. 21AYPCh. 10.3 - Prob. 3NPCh. 10.3 - Prob. 1MMCh. 10.4 - Distinguish between drug toxicity and allergic...Ch. 10.4 - Prob. 23AYPCh. 10.4 - Prob. 4NPCh. 10.4 - Prob. 5NPCh. 10 - Microbial resistance to drugs is acquired through...Ch. 10 - Prob. 2QCh. 10 - Prob. 3QCh. 10 - Prob. 4QCh. 10 - Why does the penicillin group of antibiotics have...Ch. 10 - Prob. 6QCh. 10 - Prob. 7QCh. 10 - Conduct research to find out why drugs blocking...Ch. 10 - Prob. 9QCh. 10 - Prob. 10QCh. 10 - You take a sample from a growth-free portion of...Ch. 10 - Prob. 12QCh. 10 - Treating malarial infections is theoretically...Ch. 10 - Can you think of a situation in which it would be...Ch. 10 - Prob. 15QCh. 10 - Prob. 16QCh. 10 - Prob. 17QCh. 10 - Prob. 18QCh. 10 - An antimicrobial drug with a _______ therapeutic...Ch. 10 - Prob. 20QCh. 10 - Prob. 21QCh. 10 - Prob. 1VC
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