Answered: You were tasked to check the quality of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You were tasked to check the quality of your E.coli stock from your -80 °C freezer. This isolate
will be sent in DUPLICATES to THREE different laboratories using LB slants . You need to
revive the sample in 1 tube of LB broth and inoculate it to 1 Eosin Methylene Blue plate for
quality control. From the same LB broth, you need to take a sample and inoculate it into the LB
slants that you are going to send.

Using a flow diagram, detail the steps and processes (media preparation, sterilization, inoculation) that you need to do. Show your calculations, aseptic techniques involved,
principles, and expected results. Use the product specifications of the culture media
manufactured by HiMedia or other culture media manufacturer to guide you in your media prep.

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