You were asked to perform an experiment using spectrophotometric analysis. The system that you are studying is a reaction catalyzed by the enzyme TDET and requires the coenzyme GWRM. In the reaction, substance SHARE is converted to a colored substance CRMSN that can be detected spectrophotometrically. All other substances will not absorb at the Amax of CRMSN. Test tube set-ups were prepared as follows: T1: SHARE + TDET T2: SHARE + TDET + GWRM T3: SHARE + TDET + GWRM + gradual heating until boiling T4: SHARE + TDET + GWRM + WARN* T5: TDET + GWRM *Substance WARN can bind to GWRM and prevent it from binding to TDET. QUESTION: Will product CRMSN form over time in the tubes you prepared? Complete the table below: Write a brief explanation Do you expect product CRMSN to be formed over for your answer. time? [Yes/No] T1 T2 T3 T4 T5

You were asked to perform an experiment using spectrophotometric analysis. The system that you are studying is a reaction catalyzed by the enzyme TDET and requires the coenzyme GWRM. In the reaction, substance SHARE is converted to a colored substance CRMSN that can be detected spectrophotometrically. All other substances will not absorb at the Amax of CRMSN. Test tube set-ups were prepared as follows: T1: SHARE + TDET T2: SHARE + TDET + GWRM T3: SHARE + TDET + GWRM + gradual heating until boiling T4: SHARE + TDET + GWRM + WARN* T5: TDET + GWRM *Substance WARN can bind to GWRM and prevent it from binding to TDET. QUESTION: Will product CRMSN form over time in the tubes you prepared? Complete the table below: Write a brief explanation Do you expect product CRMSN to be formed over for your answer. time? [Yes/No] T1 T2 T3 T4 T5

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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