W) Plot the "specific fluorescence” against time in a graph, and comment on the result.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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HEEELP -ME with this exersice please. 

I dont understand which values I should use for plotting the graph 

V) Calculate the "specific fluorescence" of GFP ("FL"/OD600) in samples Oh to 4h from the
induction experiment to follow how the amount of protein per cell accumulates after the
GFP synthesis has been induced. DONE
Results Table 3: Fluorescence results from the Induction experiment
Sample Fluorescence Corrected
(Flraw)
(FL)
(Flraw-Ind-B1)
Ind-Bl
Oh
1h
2h
3h
4h
6
15
78
267
816
1862
15-6=9
78-6=72
267-6-261
816-6-810
1862-6-1856
Fluorescence Correcte
d OD600
0.809
1.5
2.87
4.12
4.98
FL/ corrected OD600
(9/0.809) =11.1
(72/1.5)=48
(261/2.87) =90
(810/4.12)=196.6
(1856/4.98) =372.69
W) Plot the "specific fluorescence" against time in a graph, and comment on the result.
Transcribed Image Text:V) Calculate the "specific fluorescence" of GFP ("FL"/OD600) in samples Oh to 4h from the induction experiment to follow how the amount of protein per cell accumulates after the GFP synthesis has been induced. DONE Results Table 3: Fluorescence results from the Induction experiment Sample Fluorescence Corrected (Flraw) (FL) (Flraw-Ind-B1) Ind-Bl Oh 1h 2h 3h 4h 6 15 78 267 816 1862 15-6=9 78-6=72 267-6-261 816-6-810 1862-6-1856 Fluorescence Correcte d OD600 0.809 1.5 2.87 4.12 4.98 FL/ corrected OD600 (9/0.809) =11.1 (72/1.5)=48 (261/2.87) =90 (810/4.12)=196.6 (1856/4.98) =372.69 W) Plot the "specific fluorescence" against time in a graph, and comment on the result.
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