HEEELP -ME with this exersice please. I dont understand which values I should use for plotting the graph V) Calculate the "specific fluorescence" of GFP ("FL"/OD600) in samples Oh to 4h from theinduction experiment to follow how the amount of protein per cell accumulates after theGFP synthesis has been induced. DONEResults Table 3: Fluorescence results from the Induction experimentSample Fluorescence Corrected(Flraw)(FL)(Flraw-Ind-B1)Ind-BlOh1h2h3h4h61578267816186215-6=978-6=72267-6-261816-6-8101862-6-1856Fluorescence Corrected OD6000.8091.52.874.124.98FL/ corrected OD600(9/0.809) =11.1(72/1.5)=48(261/2.87) =90(810/4.12)=196.6(1856/4.98) =372.69W) Plot the "specific fluorescence" against time in a graph, and comment on the result.

Fluorescence is the phenomenon of emission of specific wavelengths of light by any fluorescent…

Answered: W) Plot the "specific fluorescence”…

Science

Biochemistry

W) Plot the "specific fluorescence” against time in a graph, and comment on the result.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

HEEELP -ME with this exersice please.

I dont understand which values I should use for plotting the graph

V) Calculate the "specific fluorescence" of GFP ("FL"/OD600) in samples Oh to 4h from the
induction experiment to follow how the amount of protein per cell accumulates after the
GFP synthesis has been induced. DONE
Results Table 3: Fluorescence results from the Induction experiment
Sample Fluorescence Corrected
(Flraw)
(FL)
(Flraw-Ind-B1)
Ind-Bl
Oh
1h
2h
3h
4h
6
15
78
267
816
1862
15-6=9
78-6=72
267-6-261
816-6-810
1862-6-1856
Fluorescence Correcte
d OD600
0.809
1.5
2.87
4.12
4.98
FL/ corrected OD600
(9/0.809) =11.1
(72/1.5)=48
(261/2.87) =90
(810/4.12)=196.6
(1856/4.98) =372.69
W) Plot the "specific fluorescence" against time in a graph, and comment on the result.

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