You may have heard of Dolly, the cloned sheep grown from an embryo created in a laboratory. But in the context of DNA mapping, what are clones and what essential role do they play?

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: Can you explain what semiconservative means in relationship to the way DNA is copied?

A: In the process of DNA replication, two daughter DNAs are formed with one old and one new strand…

Q: The technique of fluorescence in situ hybridization (FISH) is described. This is another method for…

A: Fluorescence in situ hybridization (FISH) is a technique used to study the genome complexity. FISH…

Q: What primers will be used to sequence your PCR product where are those sequences on your PCR product…

A: PCR is a technique used for amplification of DNA/gene of interest. At the end of the PCR technique…

Q: How many male/female sheep are needed to clone one? Cancloning be done by using adult cells?

A: One male and one female sheep is required to clone one because we need one male gamete and one…

Q: Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What…

A: PCR which stands for polymerization chain reaction is a rapid and extremely sensitive analytical…

Q: Describe the possible outcome of a PCR experiment in which (a) there is a single-stranded break in…

A: The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment…

Q: In PCR amplification Why is it important to know the length of the sequence you amplify?

A: The goal of PCR is to make enough of the target DNA region that it can be analyzed. So it is the…

Q: You are engineering a new vector that contains a screenable marker that can be used for blue/white…

A: A DNA molecule known as a cloning vector is used to introduce foreign genetic material into a host…

Q: Once the steps for RNA seq have been identified, write the steps in the order in which they are…

A: The word Transcriptome refers to the amount of total RNA transcripts in an organism. Transcriptome…

Q: Restriction enzymes are essential tools in genetic engineering. Discuss with diagrams

A: As per our guidelines, we are supposed to answer only one question. Kindly repost the other question…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.