Which is a limitation of using massively parallel sequencing ?

Q: what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the…

A: Whole - genome shotgun sequencing is a single step sequencing method, in which the genome is broken…

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: How can the concept of recombination frequency be used in genetic mapping?

A: It is the process of determining the location of genes on a chromosome that is called genetic…

Q: What is exome sequencing used for?

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: What benefits are there when using a DNA microarray over a genetic marker such as a STR?

A: The term genetic refers to the DNA molecule present in the nucleus of cells. DNA or deoxyribonucleic…

Q: When you know the sequence of the gene for the ocular protein where would you get the DNA you need…

A: The cloning is the method of recombinant DNA technology in which a piece of known DNA sequence is…

Q: Describe step 1: hybridization of template and primer in automated sanger sequencing

A: Introduction  Sanger sequencing is also known as the “chain termination method”. It is a method for…

Q: What is hierarchical sequencing?

A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…

Q: What are the possible bioethical issues that gene editing tools may encounter?

A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…

Q: What is domain? And Why is it complicated to classify and study domains purely using sequence…

A: All the prokaryotic organisms belong to Archea or Bacteria while eukaryotic organisms belong to…

Q: what kinds of techniques are used to sequence large fragments of dna upto 50kb

A: With the advancement of science and technology in the field of life sciences, there has emerged…

Q: Why are the proteins cleaved into small fragments for protein sequencing?

A: Proteins are the end product of the molecular translation that codes the information present in the…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: What does whole exome sequencing test for?

A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…

Q: How to use a microarray for transcriptome analysis ?

A: Transcriptome is the complete set of the mRNA molecules expressed in an individual . It is termed as…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: Briefly describe the applications of SNPs in DNA typing.

A: SNPs : Small nucleotide polymorphisms . It is a variation at a single position in a DNA sequence…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: Explain what is  Flow cell DNA sequencing ?

A: What is DNA sequencing? It is the process of finding out the order of nucleotides i.e. adenine,…

Q: What does massively parallel sequencing mean?

A:

Q: How are different variable region sequences generated?

A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…

Q: Mention the advantages and the challenges of using SNP markers in DNA typing

A: Advantages of using SNP markers in DNA typing: 1. The essential explanation given for thinking about…

Q: Explain how substitution matrices can be used to improve the possibility of identifying related…

A: Protein is a macronutrient that is vital for building muscle mass. It is normally found in creature…

Q: Why is GenBank important and What is GenBank format?

A: GenBank is the Genetic sequence database at NCBI (National center for biotechnology information). It…

Q: if we extract rna from a pool of cells that will that impact sequencing?

A: DNA Sequencing The process of DNA sequencing helps us determine the exact sequence of DNA that might…

Q: How many male/female sheep are needed to clone one? Cancloning be done by using adult cells?

A: One male and one female sheep is required to clone one because we need one male gamete and one…

Q: You may have heard of Dolly, the cloned sheep grown from an embryo created in a laboratory. But in…

A: Dolly is the sheep, which is considered as the first genetically modified (GMO) organism, which was…

Q: oth microarray and RNA-sequencing can study the transcriptomes. Compare microarray and RNA…

A: Microarray is based on hybridization while RNA sequencing includes cloning and sequencing.…

Q: Describe the technique of in situ hybridization. Explain how it can be used to map genes.

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: What is maxam and gilbert method of sequencing

A: DNA (deoxyribonucleic acid) sequencing is a procedure to determine the order of nucleotides…

Q: Explain the limitation of DNA microarrays ?

A: DNA microarray is the molecular laboratory technique that helps in studying and measuring the…

Q: What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein)…

A: The genetic tool CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has…

Q: How is genomic DNA prepared for shotgun sequencing?

A: Genomic DNA prepared for shotgun sequencing as follows :  1. ligating random genomic DNA fragments…

Q: What is CRISPR? Give two applications for this technology.

A: CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, is a family of…

Q: DNA polymorphisms can be used as DNA markers. Describe the differences between SNPs and STR…

A: DNA markers can help link an inherited disease with the responsible gene. the most common types of…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.