Without Inhibitor [substrate], mM 1 Rate (Vo), mM/min 0.997774599 With Inhibitor Rate (Vo), mM/min 0.847401 1.5 1.076855826 0.973852 2 1.122873947 1.037991 2.5 1.160626292 1.068431 3 1.184072485 1.105866
Without Inhibitor [substrate], mM 1 Rate (Vo), mM/min 0.997774599 With Inhibitor Rate (Vo), mM/min 0.847401 1.5 1.076855826 0.973852 2 1.122873947 1.037991 2.5 1.160626292 1.068431 3 1.184072485 1.105866
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.4P
Related questions
Question
Another member of your research group studied the kinetics of the
GAPDH from the organism. They also determined if the GAPDH from
the organism is also inhibited by the known inhibitor of GAPDH from
humans.
A. From the following data, determine the KM (Michaelis-Menten Constant) and the Vmax
(maximum velocity) of the enzyme without and with the inhibitor.
B. If GAPDH is inhibited, what specific type of inhibition is observed?
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