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QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase proteins are shown in the table below Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoele 45% 38 80% 28 65% 4 20% 98 30% 49 45% 115 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropr centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate ẞ-galactosidase? 20% O 30% 45% 65% O 80%

QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase proteins are shown in the table below Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoele 45% 38 80% 28 65% 4 20% 98 30% 49 45% 115 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropr centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate ẞ-galactosidase? 20% O 30% 45% 65% O 80%

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 45% 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 . 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate ẞ-galactosidase? 20% 30% 45% 65% O 80%
Transcribed Image Text:QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 45% 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 . 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate ẞ-galactosidase? 20% 30% 45% 65% O 80%
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