Which of the following is not a reason we often perform metagenomics studies by doing PCR using long degenerate primers for the 16S gene? Eliminates issue of having to randomly fragment DNA for sequencing (which can sometimes be tricky to optimize) All microorganisms have a 16S gene It is taxonomically informative and allows you to identify taxa using a database You can estimate relative taxa abundance by relative read abundance, avoiding genome size bias. You can make a phylogeny allowing you to do diversity analyses 16S has conserved regions and the primers used allow for some sequence variation, so we can amplify the region from different taxa efficiently using PCR
Which of the following is not a reason we often perform metagenomics studies by doing PCR using long degenerate primers for the 16S gene? Eliminates issue of having to randomly fragment DNA for sequencing (which can sometimes be tricky to optimize) All microorganisms have a 16S gene It is taxonomically informative and allows you to identify taxa using a database You can estimate relative taxa abundance by relative read abundance, avoiding genome size bias. You can make a phylogeny allowing you to do diversity analyses 16S has conserved regions and the primers used allow for some sequence variation, so we can amplify the region from different taxa efficiently using PCR
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Which of the following is not a reason we often perform metagenomics studies by doing PCR using long degenerate primers for the 16S gene?
Eliminates issue of having to randomly fragment DNA for sequencing (which can sometimes be tricky to optimize) |
||
All microorganisms have a 16S gene |
||
It is taxonomically informative and allows you to identify taxa using a database |
||
You can estimate relative taxa abundance by relative read abundance, avoiding genome size bias. |
||
You can make a phylogeny allowing you to do diversity analyses
|
||
16S has conserved regions and the primers used allow for some sequence variation, so we can amplify the region from different taxa efficiently using PCR |
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