Which of the following is/are true regarding SDS-PAGE (there may be more than one correct answer, select all that apply)? Proteins are separated based on size and charge. SDS is used to coat the proteins in a positive charge Proteins are separated based only on charge. Proteins are separated based only on size. The smallest proteins travel the farthest

Q: During SDS-PAGE,  the charge on glycine in the separating gel is ____________ while the charge on…

A: SDS-PAGE, or sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used to…

Q: Draw the structure of the following polypeptide at physiological pH. You do not need to indicate the…

A: Protein is composed of various amino acids in various proportions, which determine the structure…

Q: sodium protein

A: Macromolecules are polymers which form living cells. These are polymers and are made up of long…

Q: Which technique would be the best for analyzing lipid composition of a membrane? freeze-fracturing…

A: TLC- Chemical and immunological stains are used to find the lipids on the membrane. With the help of…

Q: During SDS-PAGE,  the charge on glycine in the stacking gel is ____________ while the charge on the…

A: SDS-PAGE is used to separate proteins based upon the relative mobility of charged species. Proteins…

Q: If you crate the absorbance spectra of a solution containing dna protein and buffer using buffer as…

A: The absorbance spectrum is formed by plotting the Absorption values against the wavelength at which…

Q: The following list of proteins are found in a mixture and are to be separated by 2D gel…

A: Two-dimensional gel electrophoresis is a modification of the regular gel electrophoresis. It is…

Q: Which of the following agents does NOT cause protein denaturation? Neutral pH Air Heat Alcohol None…

A: Introduction : Proteins are the nitrogenous organic compounds which are composed of one or more long…

Q: The graph below is a standard curve generated by plotting the distance travelled by the size…

A: The standard curve generated for an analysis involving separation of proteins along the SD-PAGE gel…

Q: Describe some of the roles proteins play in the human body.

A: A biomolecule is a carbon-based organic compound that is produced by a living organism. More than 25…

Q: Which statement about quaternary structure is TRUE?    Quaternary structure is unaffected by acids…

A: Please follow step 2 for detailed explanation.

Q: True/ False: you are asked to determine if the statement is TRUE or FALSE. You must write out the wo…

A: Separating macromolecules such as DNA, RNA, and proteins are done using gel electrophoresis.

Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size…

A: SDS-PAGE is biochemistry technique used to separate proteins based upon the relative mobility of…

Q: What characteristic of agarose makes it useful for electrophoresis?   It is more dense than water…

A: Introduction : Agarose gel electrophoresis is the most useful and common technique that is used to…

Q: Two dimensional electrophoresis separates proteins according to their isoelectric focusing and mass.…

A: Two dimensional electrophoresis:- powerful electrophoretic method for separation and analysis of…

Q: Specificity of protein/protein and protein/DNA binding is determined by what? O Non-covalent bonds O…

A: Introduction Protein, nucleic acid, lipids and carbohydrate are four biomolecules of our body.…

Q: Drag each of the following items into the corresponding boxes to indicate the protein structural…

A: Protein is a three dimensional structure formed by amino acids that polymerizes to form a…

Q: Under the microscope, the fluorescent signal from an isolated quantum point will form a stain:…

A: The fluorescence microscope is an optical microscope that uses fluorescence and phosphorescent…

Q: Select the statements that correctly describe SDS‑PAGE. SDS‑PAGE is a protein used as a loading…

A: SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a common technique used in…

Q: A microorganism was streaked onto 4 nutrient agar plates and the plates were incubated at different…

A: Nutrient agar  A type of general media that supports growth of wide variety of microorganisms.

Q: Where would you cleave to generate 2 proteins of equal size?     Question 15 options:   xi   vi…

A: Cleavage of hing region by 2-mercaptoethylamine to generate 2 proteins of equal size of protein…

Q: Refer to the figure below, what are the following forces of attraction is involved in the protein…

A: The tertiary structure of the protein is a 3D structure established and stabilised by the…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: Negatively charged proteins migrate towards the top of the gel backwards towards the positive…

A: The gel electrophoresis is a technique by which different biological samples including DNA, RNA and…

Q: The compartmentalization (division) of eukaryotic cells allows many processes to function properly.…

A: Eukaryotic cells are cells with clear defined nucleus and cell organelles have defined…

Q: Denaturation changes which protein structure(s)? SELECT ALL THAT APPLY

A: Proteins are macromolecules composed of amino acid residues. Proteins play many major role in the…

Q: This diagram shows the allowed rotations about the backbone of a peptide: Sigmoidal Curve Homology…

A: The amide group (CO-NH) is referred as peptide peptide group and has a rigid planer structure. The…

Q: The technique used to determine protein type or kind of protein in a membrane is called (type…

A: Proteins are macromolecules which are build up of long chain of amino acids joined together with…

Q: Green Fluorescent Protein has regions that are very hydrophobic. What is the meaning of…

A: The cell membrane is a structure which protects the cell and acts as a barrier and transports the…

Q: Which type of protein is classified by a polypeptide which is arranged in long strands or sheets?…

A: Each and every live cell has proteins. Protoplasm, cell membranes, and nuclear material all include…

Q: Two proteins bind the same ligand, and the data is shown below. What is the KD value for Protein B?…

A: The answer of the above question is: Correct answer not given. KD is the equilibrium dissociation…

Q: How do I organize Fibrous and Globular Protiens and what is incorrect in the picture below?

A: Characters of fibrous proteins are as follows:1. Structure is rod like2. Insoluble in water3.…

Q: Draw a UV-Vis spectrum for each protein (shown in the table below) (A, B, C, and D) from 240 – 480…

A: UV-Vis spectroscopy is a technique that measures the absorption or transmission of ultraviolet and…

Q: The primary function of chaperones is to ... Select an answer and submit. For keyboard navigation,…

A: Chaperones are the proteins that facilitate the proper folding of the proteins. These proteins are…

Q: wavelength absorption for the amino acids below from shortest wavelength to longest in the UV…

A: UV 260nm to 280nm absorption is taken as direct readout for protein concentration in a sample.…