The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Isoelectric point Protein Myoglobin Cytochrome C Molecular weight (Daltons) 17,000 7.0 10.6 12,000 150,000 IgG 6.4 Hemoglobin 64,500 5,800 66,000 15,000 33,000 7.1 Insulin 5.4 Human serum albumin 4.6 Histone H3 10.8 Pepsin 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 4 pH 7 10 direction of electric current 150 MW (KDa) 100 в E A F. G 2 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein
The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Isoelectric point Protein Myoglobin Cytochrome C Molecular weight (Daltons) 17,000 7.0 10.6 12,000 150,000 IgG 6.4 Hemoglobin 64,500 5,800 66,000 15,000 33,000 7.1 Insulin 5.4 Human serum albumin 4.6 Histone H3 10.8 Pepsin 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 4 pH 7 10 direction of electric current 150 MW (KDa) 100 в E A F. G 2 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein
Understanding Health Insurance: A Guide to Billing and Reimbursement
14th Edition
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Author:GREEN
Publisher:GREEN
Chapter6: Icd-10-cm Coding
Section: Chapter Questions
Problem 14R
Related questions
Question
match the letters of the bands to the proper protein based on the given data
![CASE 6:
The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis:
Protein
Molecular weight (Daltons)
Isoelectric point
Myoglobin
Cytochrome C
17,000
7.0
12,000
150,000
10.6
IgG
6.4
Hemoglobin
64,500
5,800
7.1
Insulin
5.4
Human serum albumin
66,000
4.6
Histone H3
15,000
33,000
10.8
Pepsin
1.0
In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their
isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via
molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller
proteins go towards the bottom.
10
pH 7
direction of electric current
150
MW (kDa)
100
в
2 4
pH 7 10
With this information, match the letters of the bands to the proper protein based on the data given.
Band Letter
Protein
wl ul](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9151c27f-e01d-4217-947a-fb7fe2567931%2F288947b6-5909-459f-bccf-5e09d5a0ea5b%2F1rcjdy_processed.png&w=3840&q=75)
Transcribed Image Text:CASE 6:
The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis:
Protein
Molecular weight (Daltons)
Isoelectric point
Myoglobin
Cytochrome C
17,000
7.0
12,000
150,000
10.6
IgG
6.4
Hemoglobin
64,500
5,800
7.1
Insulin
5.4
Human serum albumin
66,000
4.6
Histone H3
15,000
33,000
10.8
Pepsin
1.0
In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their
isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via
molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller
proteins go towards the bottom.
10
pH 7
direction of electric current
150
MW (kDa)
100
в
2 4
pH 7 10
With this information, match the letters of the bands to the proper protein based on the data given.
Band Letter
Protein
wl ul
![Band Letter
Protein
A
B
D
E
F
G
H](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9151c27f-e01d-4217-947a-fb7fe2567931%2F288947b6-5909-459f-bccf-5e09d5a0ea5b%2F9kaoi3s_processed.png&w=3840&q=75)
Transcribed Image Text:Band Letter
Protein
A
B
D
E
F
G
H
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