The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Isoelectric point Protein Myoglobin Cytochrome C Molecular weight (Daltons) 17,000 7.0 10.6 12,000 150,000 IgG 6.4 Hemoglobin 64,500 5,800 66,000 15,000 33,000 7.1 Insulin 5.4 Human serum albumin 4.6 Histone H3 10.8 Pepsin 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 4 pH 7 10 direction of electric current 150 MW (KDa) 100 в E A F. G 2 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein

The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Isoelectric point Protein Myoglobin Cytochrome C Molecular weight (Daltons) 17,000 7.0 10.6 12,000 150,000 IgG 6.4 Hemoglobin 64,500 5,800 66,000 15,000 33,000 7.1 Insulin 5.4 Human serum albumin 4.6 Histone H3 10.8 Pepsin 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 4 pH 7 10 direction of electric current 150 MW (KDa) 100 в E A F. G 2 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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