The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Isoelectric point Protein Myoglobin Cytochrome C Molecular weight (Daltons) 17,000 7.0 10.6 12,000 150,000 IgG 6.4 Hemoglobin 64,500 5,800 66,000 15,000 33,000 7.1 Insulin 5.4 Human serum albumin 4.6 Histone H3 10.8 Pepsin 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 4 pH 7 10 direction of electric current 150 MW (KDa) 100 в E A F. G 2 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein

Biochemistry
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ISBN:9781319114671
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Chapter1: Biochemistry: An Evolving Science
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match the letters of the bands to the proper protein based on the given data

CASE 6:
The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis:
Protein
Molecular weight (Daltons)
Isoelectric point
Myoglobin
Cytochrome C
17,000
7.0
12,000
150,000
10.6
IgG
6.4
Hemoglobin
64,500
5,800
7.1
Insulin
5.4
Human serum albumin
66,000
4.6
Histone H3
15,000
33,000
10.8
Pepsin
1.0
In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their
isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via
molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller
proteins go towards the bottom.
10
pH 7
direction of electric current
150
MW (kDa)
100
в
2 4
pH 7 10
With this information, match the letters of the bands to the proper protein based on the data given.
Band Letter
Protein
wl ul
Transcribed Image Text:CASE 6: The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Protein Molecular weight (Daltons) Isoelectric point Myoglobin Cytochrome C 17,000 7.0 12,000 150,000 10.6 IgG 6.4 Hemoglobin 64,500 5,800 7.1 Insulin 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 33,000 10.8 Pepsin 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 10 pH 7 direction of electric current 150 MW (kDa) 100 в 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein wl ul
Band Letter
Protein
A
B
D
E
F
G
H
Transcribed Image Text:Band Letter Protein A B D E F G H
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