What Type of guard column, separation column, and suppressor used for anion- exchange chromatography
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What Type of guard column, separation column, and suppressor used for anion-
exchange chromatography
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- Ruhemann’s purple/blue-violet coloration in Ninhydrin testQUESTION 1: What is the purpose of adding sodium hydroxide (NaOH) to the cheek cell pellet, after removing Phosphate Buffered saline, on p. 55 of the extraction protocol? Note: You might need to conduct an internet search to answer this question. The answer must be in your own words.____________ is vital to the synthesis of nucleic acids, without sufficient quantities anemia may occur.
- In your first paragraph you should explain the theory of chromatography in general, and the behind thin layer chromatography in detail. a) Define the terms "stationary phase" and "mobile phase" and describe how the stationary phase and the mobile phase interact with the analyte during a chromatographic separation (for chromatography in general – the same concept applies to thin layer, column, and gas chromatography). b) For what two main reasons would a chemist perform a thin layer chromatography analysis? c) define and describe the process of thin layer chromatography. Include the following terms: marking, spotting, developing, visualizing, and measuring. Include in your description several different ways that the plates can be visualized.Briefly describe the middle of the Ion exchanger ChromatographyThe usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?
- What is the difference between normal and reverse phase chromatography?For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.Drop rate formula