(continued) B. From a metabolic engineering perspective, why did the researchers heterologously express the adhB gene (encodes an alcohol dehydrogenase from Zymomonas mobilis (Zmo) in the

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Chapter1: Biochemistry: An Evolving Science
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QUESTION #2 (continued)
B. From a metabolic engineering perspective, why did the researchers heterologously express
the adhB gene (encodes an alcohol dehydrogenase from Zymomonas mobilis (Zmo) in the
strain when the adhE gene (encodes the native E. coli alcohol dehydrogenase) is already
endogenously expressed?
C. "Ethanol production is decreasing after 75 hours because it is being converted into succinate"
Do you agree with this interpretation of the data? Explain your answer.
D. "By 110 hours 100% of the sugars in the medium had been consumed." Do you agree with
this interpretation of the data? Explain your answer.
Transcribed Image Text:QUESTION #2 (continued) B. From a metabolic engineering perspective, why did the researchers heterologously express the adhB gene (encodes an alcohol dehydrogenase from Zymomonas mobilis (Zmo) in the strain when the adhE gene (encodes the native E. coli alcohol dehydrogenase) is already endogenously expressed? C. "Ethanol production is decreasing after 75 hours because it is being converted into succinate" Do you agree with this interpretation of the data? Explain your answer. D. "By 110 hours 100% of the sugars in the medium had been consumed." Do you agree with this interpretation of the data? Explain your answer.
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