QUESTION 24What can be done to increase the rate at which a protein of interest moves down an ion-exchange chromatographycolumn?Add a small amount of a non-ionic detergents to the eluant.Change the pH of the eluant.Reduce the ion concentration in the eluantReduce the temperature of the eluant.Add a protease inhibitor to the eluant.

Proteins are polymers of amino acids. The sequence of amino acids determines the function, physical…

Answered: What can be done to increase the rate…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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If a sample is composed of a mixture of maltose, maltodextrin, and glucose, which of the following is expected? Adding alkaline CuSO4 to the solution will turn the solution into orange with some dark red precipitate. Performing Molisch Test on the sample will give a negative result. Reaction of the sample with phenylhydrazine will give a solution with yellow precipitate. Furthermore, inspection of the precipitate under the microscope will show cotton ball shaped crystals. . The Osazone Test on the sample will give a yellow solution with no visible precipitate.
Angiotensin Converting Enzyme (ACE) is the target of ACE inhibitors such as captopril that are used therapeutically to control blood pressure. The enzyme ACE converts angiotensin I to angiotensin II. Angiotensin II is an octapeptide with the sequence Asp-Arg-Val-Tyr-Ile-His-Pro-Phe. What will its net charge be at pH 7? A) -2 B) -1 O C) O D) +1 E) +2
Fill the table given the info below. Please thank you. Protein at 1 mg/mL which is also 1g/L. Use the DNA sequence below to export to ExPASy Translate & ProtParam. (I tried doing so and I calculated a Molarity of 16.51uM which came from the 60,567.28Da from ExPASy). Using the molarity then use it to fill out the table.…
Consider the decapeptide angiotensin I.a. What products are formed when angiotensin I is treated with trypsin?b. What products are formed when angiotensin I is treated with chymotrypsin?c. Treatment of angiotensin I with ACE (the angiotensin-converting enzyme) cleaves only the amide bond with the carbonyl group derived from phenylalanine to afford two products. The larger polypeptide is angiotensin II, a hormone that narrows blood vessels and increases blood pressure. Give the amino acid sequence of angiotensin II using three-letter abbreviations. ACE inhibitors are drugs that lower blood pressure by inhibiting the ACE enzyme
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You run 5 standard proteins listed below on a size-exclusion (gel filtration) column with limit of 200,000Da. Please draw a chromatogram on a separate page with each peak and both axes labeled. Protein B-Amylase Alcohol dehydrogenase Bovine albumin Carbonic anhydrase Cytochome A280 MW 223,800 0.5 82,000 0.6 66,463 0.45 30,000 0.43 12,000 0.8
Consider the proteins in the table below. Protein Isoelectric point (pl) М.W. Alcohol dehydrogenase 150,000 5.5 Cytochrome C 12,400 10.5 Serum albumin 66,000 5.3 B-Amylase 200,000 5.7 Apoferitin 443,000 4.0 Fill in the blank. If the mixture of proteins above was subjected to anion exchange chromatography at pH 8, from the column. would be the first protein to elute
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Determine the two true statements below about this experiment. Milk is an acidic solution. Milk is a heterogenous solution. The isoelectric point (pl) is when a protein has a large overall positve charge. Casein is the primary saccharide in cow's milk.
Are Xanthoproteic and MillonNasse tests satisfactory for use in the urinary examination for protein? Why? Which test can be used to show up to what stage the hydrolysis of a protein proceeds? Why?
Please describe the protein purification process with the aim of purifying a protein which locates on nucleus membrane.

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