what are the possible probes of a 15- nucleotide length that would be used in a PCR reaction that could introduce a codon change to the amino acid substitution. Which one should be used and why?

Human Anatomy & Physiology (11th Edition)
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1. what are the possible probes of a 15- nucleotide length that would be used in a PCR reaction that could introduce a codon change to the amino acid substitution. Which one should be used and why? 

 

GCC AUG AGC CUU AUU UUU AAA.
Ala Met Ser Leu Ilu Phe Lys
Transcribed Image Text:GCC AUG AGC CUU AUU UUU AAA. Ala Met Ser Leu Ilu Phe Lys
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Probes are fluorescently labelled DNA oligonucleotides used in PCR. They are designed in such a way that they may bind to one of the primers during the PCR reaction. Binding  gives a fluorescent signal during the reaction. 

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