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We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c?

We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c?

esday, June 9, 2021, most features of Word will be disabled. Reactivate In the oxidase test we will determine which bacteria have cytochrome c, one version of the important enzyme in the electron transport chain,. This enzyme plays a critical role in all those reaction with NADH, however some bacteria use alternative cytochromes. Thus, this becomes another way to distinguish between species. Lastly, we will conduct the catalase test. As mentioned in lecture, during respiration some of the hydrogen may combine with oxygen to form hydrogen peroxide, H2O2. Hydrogen peroxide is lethal to many bacteria. To protect themselves, they produce the enzyme catalase to break it down to water and oxygen. We will determine which bacteria produce this useful enzyme. 1. When nitrate is used as the final electron acceptor, what two possible compounds might it be reduced to? 2. What reagent is added to culture in nitrate broth to determine if nitrite is present? 3. What does a positive test with zinc dust mean for nitrate media? 4. We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c? 5. When liquid hydrogen peroxide is dropped on the bacterial colonies for the catalase test, what gas is produced that causes the bubbles? A Display Settings Focus
Transcribed Image Text:esday, June 9, 2021, most features of Word will be disabled. Reactivate In the oxidase test we will determine which bacteria have cytochrome c, one version of the important enzyme in the electron transport chain,. This enzyme plays a critical role in all those reaction with NADH, however some bacteria use alternative cytochromes. Thus, this becomes another way to distinguish between species. Lastly, we will conduct the catalase test. As mentioned in lecture, during respiration some of the hydrogen may combine with oxygen to form hydrogen peroxide, H2O2. Hydrogen peroxide is lethal to many bacteria. To protect themselves, they produce the enzyme catalase to break it down to water and oxygen. We will determine which bacteria produce this useful enzyme. 1. When nitrate is used as the final electron acceptor, what two possible compounds might it be reduced to? 2. What reagent is added to culture in nitrate broth to determine if nitrite is present? 3. What does a positive test with zinc dust mean for nitrate media? 4. We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c? 5. When liquid hydrogen peroxide is dropped on the bacterial colonies for the catalase test, what gas is produced that causes the bubbles? A Display Settings Focus
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