Q: What is different between Benedict test that use for qualitative determination and quantitative…
A: Glucose is the monosaccharide carbohydrate that has a free carbonyl group i.e. aldehyde which makes…
Q: What would be the expected out come of a triple sugar iron test If the bacteria is a pathogenic…
A: TRIPLE SUGAR IRON TEST 1.The Triple Sugar Iron test is a microbiological test having ability to test…
Q: Between Enterobacter aerogenes and Enterobacter cloacae, which are positive or negative for methyl…
A: methyl red and Voges Proskauer tests both are biochemical tests for identifying bacteria in…
Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli
A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…
Q: Why is hydrolysis of sucrose also known as specific sucrose test?
A: Hydrolysis is a chemical reaction where a molecule of water ruptures one or more chemical bonds. The…
Q: What would be the expected out come of a phenol red test If the bacteria is a pathogenic intestinal…
A: Phenol Red Broth is a general-purpose differential test medium typically used to differentiate…
Q: What would be the expected out come of a Nitrate reduction test If the bacteria is a pathogenic…
A: Nitrate Reduction Test is used to determine the ability of an organism to reduce nitrate (NO3) to…
Q: Organism 1 Organism 2
A:
Q: What is the media used in Catalase test and its expected results?
A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…
Q: If coliforms were present on a plated sample, would you know with certainty that the coliforms are…
A: Coliform microbes are characterized as rod-shaped, gram-negative, non-spore-forming, and…
Q: Need help Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.
A: INTRODUCTION How to make a pure culture from mixed culture is explained below.
Q: Can the resazurin reduction test be used to determine whether a milk is properly pasteurized or…
A: Pasteurization is a process that helps in keeping the milk fresh and helps in avoiding the…
Q: Why are nitrates added to cured meats?
A: Nitrates can be defined as the set of compounds that are indulged in nitrogen and oxygen molecules.…
Q: how do microorganisms such as bacteria eliminate harmful reactive oxygen species?
A: Reactive oxygen species (ROS) are emerging as important elements in the bacterial response to lethal…
Q: Between Proteus mirabilis and Proteus vulgaris which are positive or negative for ornithine…
A: ornithine decarboxylase test is a special kind of biochemical test that helps to determine whether…
Q: What is the EFFECT and RATIONAL of Molten agar mixed with bacterial suspension has temperature…
A: Bacteria can be cultured in either a liquid media or a solid media based on the purpose of culturing…
Q: Why do the results for the confirmed test represent faecal coliform in the analysis of water using…
A: MPN is Most probable number. It is commonly applied for testing the quality of water.
Q: What effect does the H2O2 have on bacteia in the catalase test?
A: The production of toxic substances like hydrogen peroxide due to metabolic activities can be lethal…
Q: In the multiple fermentation tube technique does the absence of gas formation after 24 hours…
A: Coliform microbes are life forms that are available in the climate and in the defecation of all…
Q: Between Enterobacter aerogenes and Enterobacter cloacae, which are positive or negative for…
A: The result is shown below.
Q: what does it mean when bubbles shows during a catalase test
A: Bacteria are a group of prokaryotic microscopic single celled organisms. They live in diverse…
Q: Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?
A: Colony-forming unit (CFU) represents the number of colonies formed on the media plate from one…
Q: What is the principle involved in the Acrolein test? What is its purpose?
A: Qualitative tests are the chemical or physical tests involved in identification of certain…
Q: What are the characteristics of an effective indicator organism? Write down some examples. Suppose…
A: Characteristics: It should have the same resistance to environmental stress as that of pathogens. It…
Q: does ribose-5-phosphate pass fehlings test?
A: Fehling's test is one of the sensitive test for detection of reducing sugars. Fehling's solution is…
Q: Is tripple sugar iron test a efficient test for unknown intestinal bacteria?
A: Triple sugar iron agar is a differential medium that contains lactose, sucrose, a small amount of…
Q: Which statement is supported by the experiment below? Select all the apply. 1% NaCl agar plate 6.5%…
A: Here, both S.aureus and E.coli can grow in normal salt concentration S.aureus can tolerate…
Q: If an esculinase-postive and an esculinase-negative bacteria species can both grow on the Bile…
A: Bile Esculin Agar It is defined as the type of selective differential agar that's used to isolate…
Q: Which of the following tests can be used to distinguish between Enterobacter and Pseudomonas…
A: Introduction Small, single-celled organisms are known as bacteria. Nearly every place on Earth…
Q: Why is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar…
A: Brewer’s anaerobic agar and fluid thioglycollate medium (FTM) are used to grow bacteria. Both of…
Q: What is the coliform test when is it used? What is its purpose? How does it function? What are the…
A: All water that is naturally exposed to the atmosphere, such as rivers, lakes, and reservoirs. It is…
Q: In a Triple Sugar Iron test, what are the results and (what should they look like) for the bacteria:…
A: triple sugar ion test or TSI is a confirmatory as well as a differentiative biochemical test which…
Q: What is the importance of Acrolein Test? What component in fats and oil will respond to the test?
A: The Acrolein testing is performed on a 'dehydration reaction' in which the water molecules are…
Q: Which test is used to differentiate between fermentative and oxidative metabolism? Describe the…
A: before we can go for the specific test required, let's first understand the difference between…
Q: What is the substrate(s) that will be used by the microorganism to produce the calcite ?
A: Biomineralization is the process by which microorganisms and other living organisms directly or…
Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…
A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…
Q: in the isolation of alkaloid,in the early stages of acid or base required conditions. explain the…
A: Alkaloids are secondary metabolites derived through shikimic acid pathway. They are nitrogen…
Q: In the Mannitol Salt Agar, why is there no observed growth of Escherichia coli?
A: In microbiology, mannitol salt agar is a typical growth media. It promotes the development of one…
Q: Do the results from a confirmed coliform test represent total or faecal coliform and why?
A: Fecal coliform bacteria may occur in ambient water as a result of the overflow of domestic sewage or…
Q: operties of the Snyder deep media allow us to detect susceptibility to dental caries?
A: Dental caries (tooth decay) is injury to enamel of tooth that be converted into little holes or…
Q: What kinds of organisms may be plentiful in a milk sample, yet give a negative reductase test?
A: Methylene blue reduction test is based on the principle of oxygen reduction by increased microbial…
Q: Other than the PYR test, what is another use of the PEP reagent?
A: PYR is a colorimetric method used to detect the presence of pyrolidonyl arylamidase activity in a…
Q: why are methanol, acetone, and 2-propanol quantified for BAC determinations
A: Blood Alcohol Concentration BAC is a technique that is used to measure alcohol content in the…
Q: If testing had come back as oxidase negative, urease positive and growth on MacConkey agar, what…
A: MacConkey agar is a selective and differential culture medium used for the isolation and…
Q: In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test…
A: The process of chemical breakdown of complex substances into simple ones in the absence of oxygen…
Q: If the seven-digit profile that is tabulated cannot be found in the API® 20E Analytical Profile…
A: The API® 20E System, is a biochemical panel for identifying and differentiating members of the…
Q: Clear tubes in the MIC test were inoculated in Mueller-Hinton Agar to determine the MBC of the…
A: MBC - stands for minimum bactericidal concentration It is the lowest concentration of antibiotics…
Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…
A: Answer : First step of the kirby-Bauer test :-
Q: Which of the characteristics identified using SIM media, which would be most useful in…
A: SIM agar media is referred to as a sulfur or indole or motility test combined in one agar deep if…
can the phenol red test also be used to determine if a certain bacterium can metabolze various carbohydrates?
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- Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.What is the logic behind testing for E. coli to detect fecalcontamination of water?If the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitative
- Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.Why does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?Does Escherichia coli provide positive or negative results for the lysine decarboxylase and ornithine decarboxylase test results?
- Will disaccharides and polysaccharides give positive results in Molisch test? How?Why does the oxidase test have to be read quickly?Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer. In addition to H2S production and motility, for what other test can SIM medium be used?
- A mistake was made in preparing the arginine decarboxylase test, and mineral oil was NOT added to the test tube after inoculating with bacteria. If an arginine decarboxylase-positive bacteria species was inoculated into the above arginine-decarboxylase broth without mineral oil, what would likely be the color result of this bacteria in this test? Would this produce a false positive or false negative result? Explain.What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What is the media used? What is the product of this test that reacts with the reagent to give positive result? What biochemical abilities do these organisms have (or lack)?In a Triple Sugar Iron test, what are the results and (what should they look like) for the bacteria: Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhimurium, and Serratia marcescens?