Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 a. Calculate the retention factor for each solute b. Calculate the selectivity factor c. How many plates are needed to provide a resolution of 1.5? d. What column length should be used to provide a resolution of 1.5 if the plate height of the packing is 1.5 ×10-3 cm? e. If the flow rate is 6.75 cm min-1 , how long will it take to elute the two species? (the question wanted to be answered)
Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 a. Calculate the retention factor for each solute b. Calculate the selectivity factor c. How many plates are needed to provide a resolution of 1.5? d. What column length should be used to provide a resolution of 1.5 if the plate height of the packing is 1.5 ×10-3 cm? e. If the flow rate is 6.75 cm min-1 , how long will it take to elute the two species? (the question wanted to be answered)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16.
They are separated by elution on silica gel with hexane as eluent. The ratio for the packing
VS/VM =0.425
a. Calculate the retention factor for each solute
b. Calculate the selectivity factor
c. How many plates are needed to provide a resolution of 1.5?
d. What column length should be used to provide a resolution of 1.5 if the plate height of
the packing is 1.5 ×10-3
cm?
e. If the flow rate is 6.75 cm min-1
, how long will it take to elute the two species? (the question wanted to be answered)
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