Explain what will happen when you use 50 microgram of an analyte in a chromatography column which has 3 theoretical plates. The partition coefficient in this setup is 1/4. You must explain the subsequent distribution of the analyte as it moves through the three theoretical plates
Explain what will happen when you use 50 microgram of an analyte in a chromatography column which has 3 theoretical plates. The partition coefficient in this setup is 1/4. You must explain the subsequent distribution of the analyte as it moves through the three theoretical plates
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Explain what will happen when you use 50 microgram of an analyte in a chromatography column which has 3 theoretical plates. The partition coefficient in this setup is 1/4. You must explain the subsequent distribution of the analyte as it moves through the three theoretical plates
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Step 1
The partition coefficient can be expressed as:
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