The results of the diagram (Agarose electrophoresis photodocumentation andquantification table) correspond to the information for 4 samples of genomic DNAextracted from plant tissue. With which sample (s) could a PCR be performed toamplify a 100 pb region?Al A2АЗ А4IDDNA260 nm 260 nm(pb)10,000sample (ng/uL) 280 m 230 nmA11701.92.0А21801.51.55,000АЗ1701.71.5А41701.92.01,000500100choose the best option:а. Allb. Noneс. А2d. A1, A2е. А1

Agarose gel electrophoresis is the separation technique based upon the size of the macromolecule. in…

Answered: The results of the diagram (Agarose…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Think about the function of the primers in a PCR that is designed to amplify a specific sequence, and about the temperatures of the PCR cycle. After considering these two points, give two reasons why 4-nucleotide long primers are not appropriate for PCR and explain.
If a researcher attempted to do a PCR amplification of the slc24a5 gene in the gol1 mutatant line, would their attempts have been successful? Why or why not? No; PCR amplification will not be successful because the gene is deleted in the golb1 mutatant line. No; PCR amplification will not be successful because there is a point mutation in the coding region of the gene. Yes; this mutant line had a point mutation that would not have impacted PCR. Yes; although the gene switch was mutated, it did not impact the promotor sequence. O O O O
The exponential nature of PCR allows spectacular increases in the abundanceof a DNA sequence being amplified. Consider a 10-kbp DNA sequence in agenome of 1010 base pairs. What fraction of the genome does this sequence represent? That is, what is the fractional abundance of this sequence in this genome?Calculate the fractional abundance of this target sequence after 10, 15, and 20 cycles of PCR, starting with DNA representing the whole genome and assuming that no other sequences in the genome undergo amplification in the process.
Answer the question in the photo
1. Primers come at a concentration of 200 pmol//ul (uM). You want the final concentration of your primers to be 0.2 uM. How would you dilute the primers in water so that you can add 1 ul in a PCR mix with a total volume of 50ul?
Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind to the DNA strands at a low temperature. PCR also preferentially amplify short segments of DNA. Would it be important to actually run the cDNA after the PCR on a DNA gel in order to check for a PCR product of the predicted size for the insulin gene? Why or why not? How many more genes in the plasmid (besides the insulin cDNA insert) are need to determine which bacterial cells have been transformed and to determine which transformed bacterial cells have a plasmid with the cDNA insulin insert? What is needed in the plasmid with the cDNA insert besides the gene for insulin to actually cause the bacteria to express the insulin gene and produce the insulin protein?
What are five variables to consider when designing a primer for standard PCR and state why.
In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI
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The gel below shows results for the bitter tasting SNP analysis from a CH306 class from a few years ago. Analyze the results and annotate the gel to indicate the bitter tasting ability and homozygous/heterozygous status of each of the individuals represented on the gel. There are three lanes of markers, and the first non-maker lane on the left of the gel is an uncut control PCR product (i.e. there are a total of 10 individuals on the gel starting in lane 3).
Why does DTT enhance the PCR reaction?( DTT causes a problem).
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The results of the diagram (Agarose electrophoresis photodocumentation and quantification table) correspond to the information for 4 samples of genomic DNA extracted from plant tissue. With which sample (s) could a PCR be performed to amplify a 100 pb region?