The muscle isozyme of lactate dehydrogenase is inhibited by lactate. Steady state kinetic analysis yielded the following data, with lactate either absent or present at a fixed concentration. (a) Pyruvate is the substrate whose concentration is varied in one plot, NADH in the other. Identify each. Use an arrow and the appro- priate letter (b, c, d, or e) to identify each of the following. (b) Reciprocal of Vmax for the uninhibited enzyme. (c) The line representing data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate. (d) The line representing data obtained in the presence of lactate acting as a mixed inhibitor with respect to the variable substrate.
The muscle isozyme of lactate dehydrogenase is inhibited by lactate. Steady state kinetic analysis yielded the following data, with lactate either absent or present at a fixed concentration. (a) Pyruvate is the substrate whose concentration is varied in one plot, NADH in the other. Identify each. Use an arrow and the appro- priate letter (b, c, d, or e) to identify each of the following. (b) Reciprocal of Vmax for the uninhibited enzyme. (c) The line representing data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate. (d) The line representing data obtained in the presence of lactate acting as a mixed inhibitor with respect to the variable substrate.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![The muscle isozyme of lactate dehydrogenase is inhibited by lactate.
Steady state kinetic analysis yielded the following data, with lactate
either absent or present at a fixed concentration.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcc6bfc5e-a79d-4d57-905c-7fb9a6e63596%2F42b5c234-9d4f-444e-af0c-dd1dedcfd1b2%2Ffklxex_processed.png&w=3840&q=75)
Transcribed Image Text:The muscle isozyme of lactate dehydrogenase is inhibited by lactate.
Steady state kinetic analysis yielded the following data, with lactate
either absent or present at a fixed concentration.
![(a) Pyruvate is the substrate whose concentration is varied in one
plot, NADH in the other. Identify each. Use an arrow and the appro-
priate letter (b, c, d, or e) to identify each of the following.
(b) Reciprocal of Vmax for the uninhibited enzyme.
(c) The line representing data obtained in the presence of lactate
acting as a competitive inhibitor with respect to the variable
substrate.
(d) The line representing data obtained in the presence of lactate
acting as a mixed inhibitor with respect to the variable substrate.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcc6bfc5e-a79d-4d57-905c-7fb9a6e63596%2F42b5c234-9d4f-444e-af0c-dd1dedcfd1b2%2Fhod1kbi_processed.png&w=3840&q=75)
Transcribed Image Text:(a) Pyruvate is the substrate whose concentration is varied in one
plot, NADH in the other. Identify each. Use an arrow and the appro-
priate letter (b, c, d, or e) to identify each of the following.
(b) Reciprocal of Vmax for the uninhibited enzyme.
(c) The line representing data obtained in the presence of lactate
acting as a competitive inhibitor with respect to the variable
substrate.
(d) The line representing data obtained in the presence of lactate
acting as a mixed inhibitor with respect to the variable substrate.
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