The GAL4 expression system can be used to experimentally induce expression of a gene in a specific tissue at a specific time. You want to study the overexpression of Your Favorite Gene (YFG) in mouse ear development. However, if you overexpress this gene everywhere, the mouse is not viable past early embryo development. You have the following mouse lines with different mutations or transgenic constructs available. Which two will you cross together to answer your research questions? GAL4 with constitutive enhancer GAL4 with embryo enhancer GAL4 with ear enhancer O yfg loss-of-function line YFG CDNA with UASG promoter YFG CDNA with constitutive promoter

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Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.