The following table are the results of spectrophotomety of extracted RNA samples (note: notall OD's reported in this table are relevant for all subsequent questions).Specrophotometry- RNA ExtractionsExperimentalGroupReplicate O0260OD280OD3200036010.680.380.700.7321.350.751461.381.851.031.931.9610.680.380.800.8521801.001.961.980.960.531.111.18What is the SSRNA concentration for sample A1?Select one:O a 273 ug/mlO b. 18.9 ug/mlOc 34.1 ug/mlO d 15.1 ug/mlO e 22.5 ug/ml

DNA is usually double-stranded unless mentioned for certain viruses. RNA is usually a…

Answered: The following table are the results of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Note: Answer the number 3 question only. Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.
The table shows the presence/absence and size of DNA bands of known samples using gel electrophoresis. Explain conclusions you can draw from the data in the table.
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828.
You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average molecular weight of a deoxynucleotide monophosphate is 328 g/mol.
Bacterial mutants can be identified in screens or selections.In a screen, individual colonies are tested for a particularphenotype. In a selection, only bacteria with the phenotypein question are recovered as colonies, enabling theidentification of extremely rare _________.
Match the PCR sample with the predicted result on the agarose gel. 1) Sample from individual homozygous for their PV92 locus with the Alu insert. Five bands of 100, 200 500, 700 and 1000bp Single band of 300 bp Two bands one of 300 bp and one of 641bp Tow bands one of 641 bp and one of 941 bp No Bends Single band of 941 bp Single band of 641bp 2) Sample from individual homozygous for their PV92 locus without the Alu insert. Five bands of 100, 200 500, 700 and 1000bp Single band of 300 bp Two bands one of 300 bp and one of 641bp Tow bands one of 641 bp and one of 941 bp No Bends Single band of 941 bp Single band of 641bp 3) Sample from individual heterozygous for their PV92 locus having the Alu insert. Five bands of 100, 200 500, 700 and 1000bp Single band of 300 bp Two bands one of 300 bp and one of 641bp Tow bands one of 641 bp and one of 941 bp No Bends Single band of 941 bp Single band of 641bp 4) Negative control sample. Five bands of 100, 200 500, 700 and 1000bp…
Give an estimate of the fragment sizes (basepairs) of the DNA bands from each sample lane of the gel electropherogram below. lane 1: lane 2: lane 3:
Explain how the spin columns were effective in collecting the isolated RNA from the sample and why adding water eluted the RNA into the tube for PCR diagnostic
The results of the diagram (Agarose electrophoresis photodocumentation and quantification table) correspond to the information for 4 samples of genomic DNA extracted from plant tissue. With which sample (s) could a PCR be performed to amplify a 100 pb region? Al A2 АЗ А4 ID DNA 260 nm 260 nm (pb) 10,000 sample (ng/uL) 280 m 230 nm A1 170 1.9 2.0 А2 180 1.5 1.5 5,000 АЗ 170 1.7 1.5 А4 170 1.9 2.0 1,000 500 100 choose the best option: а. All b. None с. А2 d. A1, A2 е. А1
DNA samples were then run in agarose gel electrophoresis against a molecular standard of 100 bp and 1Kb Ladder. The figure is analyzed and labelled manually and recorded with the used product ladder information. Figure 2A is labeled with corresponding lane numbers and with M1 and M2 as markers used. Write an interpretation of the documented gelresult (Figure 2). Note: Agarose gel of isolated DNA (A) from plant (lanes 1 & 2), chicken (lanes 3 & 4), and E. coli (lanes 5 & 6) with Vivantis* product information of 100bp (B) and 1kb ladders (C).
Please answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24. As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain. Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an…
Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? ddATP ddGTP ddCTP ddTTP - GGTTACC B CCATTGG CCAATGG GGTAACC | | | |

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