Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following: Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample). The streak plate was generated using 4 areas (quadrants)- A, B, C & D. Area A was inoculated with a loop containìng 3 mìllion organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C & D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained). What is the expected outcome in Area on this streak plate? 2d

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider
the expected outcome on the streak plate after the following:
Two organisms were in the original sample. Organism G was present at twice the
abundance of Organism H (so 2/3 of the original sample).
The streak plate was generated using 4 areas (quadrants)- A, B, C & D. Area A was
inoculated with a loop containìng 3 mìllion organisms from the original culture, which
was mixed very well and considered to be homogeneous. Areas B, C & D were streaked
from the previous area with a sterilized loop, with each streak transferring 2% of the
organisms from the previous area (assume the relative abundance of the two organisms
was maintained).
What is the expected outcome in Area
on this streak plate? 2d
Transcribed Image Text:Performing a bacterial isolation by streaking, using sterile (aseptic) technique, consider the expected outcome on the streak plate after the following: Two organisms were in the original sample. Organism G was present at twice the abundance of Organism H (so 2/3 of the original sample). The streak plate was generated using 4 areas (quadrants)- A, B, C & D. Area A was inoculated with a loop containìng 3 mìllion organisms from the original culture, which was mixed very well and considered to be homogeneous. Areas B, C & D were streaked from the previous area with a sterilized loop, with each streak transferring 2% of the organisms from the previous area (assume the relative abundance of the two organisms was maintained). What is the expected outcome in Area on this streak plate? 2d
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