The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m 6- RNA Absorbance at 260 (µg) 0.00 10.29 20.58 30.87 41.16 mass= nm 0.227 0.452 0.786 1.078 1.296 1g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. ug
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m 6- RNA Absorbance at 260 (µg) 0.00 10.29 20.58 30.87 41.16 mass= nm 0.227 0.452 0.786 1.078 1.296 1g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. ug
ChapterU5: Fire: Energy , Thermodynamics, And Oxidation-reduction
SectionU5.116: How Absorbing: Spectroscopy
Problem 6E
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Question
![The data in the table are used to create a calibration curve for the determination of RNA from
its absorbance at 260 nm.
Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the
best straight line fit to the calibration curve. Do not subtract the blank reading when creating
the calibration curve.
m=
RNA
(µg)
0.00
10.29
20.58
30.87
41.16
mass=
Absorbance at 260
nm
0.227
0.452
0.786
1.078
1.296
Hg-1
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of
0.523.
µg](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F80d908ba-ee14-420f-a1e1-d6eb26f3e191%2Fe200680b-313c-4e32-bdbf-3748531db90c%2Fxpv7cws_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The data in the table are used to create a calibration curve for the determination of RNA from
its absorbance at 260 nm.
Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the
best straight line fit to the calibration curve. Do not subtract the blank reading when creating
the calibration curve.
m=
RNA
(µg)
0.00
10.29
20.58
30.87
41.16
mass=
Absorbance at 260
nm
0.227
0.452
0.786
1.078
1.296
Hg-1
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of
0.523.
µg
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