You obtained the following raw data when setting up a Bradford standard curve:BSA (mg/ml) Absorbancy595nm00.17810.27720.39530.47540.611756989d100.7210.8540.9311.0411.0591.087Set up a standard curve.What would the quality of the line-fit (R² value) be if you exclude experimental outliers?(Give you answer to 4 decimal places)

The provided data is as follows:BSA (mg/ml)Absorbance (595…

Answered: You obtained the following raw data…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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J 5
Chemistry
A chemist received a report from an analysis involving the iron-phenanthroline method, containing the calibration curve for Fe standards (20 ppm, 30 ppm, 40 ppm, 50 ppm, and 60 ppm) and their corrected absorbances. The report stated that the equation of the line and r² were as follows: y = 0.015066 x -0.154424 r² = 0.9997 The sample they tested gave a corrected reading of 0.417. How much Fe is present in ppm? (A) 2.80 B) 37.93 17.43 D) 2.60
Calculate the path length in centimetres which would give an absorbance of 0.134 of a colored dye with a molar absorptivity of 0.759 L mol-1 cm-1 at a wavelength of 645 nm if the concentration of the dye is 6.82x10-2 M. Report your answer to 3 significant figures.
2) In a laboratory study, a calibration was performed using NH4C1 to analyze ammonium spectrophotometrically. The results of the study are given below. According to this, when the 5.0 mL sample containing ammonium is completed to 50 mL with pure water, find the amount of NH4 in the sample whose absorbance was found as 0.12 according to the table. (N: 14 g / mol, H: 1 g / mol, Cl: 35.5 g / mol). Concentration (mg/L) Absorbance 0.01 5 0.05 7.5 0.1 10 0.15 12 0.18
The absorbance for a dye sample of unknown concentration is measured and the corrected absorbance is 0.666. The standard curve created is found to have this linear equation: y = 0.153x + 0.043 Calculate the concentration (ug / mL) of the dye sample. 4.54 3.33 4.07 5.17 2.07
1
Hh.130.
2. ] You make 150.0 mL of a copper(II) sulfate solution but forget to cover it before leaving for the day. The next class, you measure the volume and find that you only have 132.0 mL remaining. You didn’t record the initial concentration, but you measure the absorbance of the remaining solution at 620 nm and find that it is 0.386. You also construct the following calibration curve for CuSO4: Standard solution Abs620 0.50 M 0.424 0.40 M 0.336 0.30 M 0.247 0.20 M 0.159 (refer to image) a. What is the value for k (in Beer’s Law)? Provide a calculation or graph to support your answer. b. What is the concentration of the (remaining) solution? c. What was the concentration of the original solution from the first class? d. If you removed 7.5 mL from the 132.0 mL solution in the process of measuring the absorbance, how much water should you add to obtain the original concentration that you solved for in part (c)?
Given the calibration curve below, what is the limit of linearity? Absorbance 2000 1800 1600 1400 1200 1000 800 600 400 200 0 Ο 0.2 μg/ml Ο 0.4 μg/ml Ο 0.8 μg/ul Ο 1.2 μg/ul 0 Non-linear regresssion ...... 1 Concentration µg/μl 0.2 0.4 0.6 0.8 R²=0.9966 1.2 1.4 1.6
explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.155 1 0.287 2 0.420 3 0.523 4 0.651 5 0.756 6 0.878 7 0.918 8 0.954 9 10 0.982 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.566 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 decimal places).

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