6. The UV-Vis absorption spectrum was measured for three different concentrations (10 mM, 20 mM, and 30 mM) of an analyte that we will call orangium as shown in the figure below. A 1.000 cm cuvette is used for all of the experiments. 1.00 0.90 0.80 0.70 0.60 a 0.50

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6. The UV-Vis absorption spectrum was measured for three different
concentrations (10 mM, 20 mM, and 30 mM) of an analyte that we will call
orangium as shown in the figure below. A 1.000 cm cuvette is used for all of
the experiments.
1.00
0.90
0.80
0.70
0.60
a
0.50
<D.40
0.30
0.20
0.10
0.00
280
1
1
330
380
---Absorbance (30 mM)
-Absorbance (20 mM)
Absorbance (10 mm)
430
480
530
580
Wavelength (nm)
630
680
730
780
calcolate
a.) Identify the best wavelength (by drawing a vertical line on the spectrum)
use when doing absorption spectroscopy experiments that measure the
concentration of orangium. Give me a couple of reasons why this
wavelength is a good choice.
Transcribed Image Text:6. The UV-Vis absorption spectrum was measured for three different concentrations (10 mM, 20 mM, and 30 mM) of an analyte that we will call orangium as shown in the figure below. A 1.000 cm cuvette is used for all of the experiments. 1.00 0.90 0.80 0.70 0.60 a 0.50 <D.40 0.30 0.20 0.10 0.00 280 1 1 330 380 ---Absorbance (30 mM) -Absorbance (20 mM) Absorbance (10 mm) 430 480 530 580 Wavelength (nm) 630 680 730 780 calcolate a.) Identify the best wavelength (by drawing a vertical line on the spectrum) use when doing absorption spectroscopy experiments that measure the concentration of orangium. Give me a couple of reasons why this wavelength is a good choice.
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