Use the calibration curve below to calculate the concentration in uM of phenol red in a sample with an absorbance at 550 nm of 0.109 *Give your answer to 3 significant figures and do not include the units (UM) in the answer. Abs orbance (550 nm) 0.160 0.340 0.120 0.100 0.060 0.040 0.020 0.000 0.0 2.0 y = 0.0145x + 0.0028 R = 0.9847 4.0 6.0 [Phenol Red] (M) 8.0 10.0 12.0

Use the calibration curve below to calculate the concentration in uM of phenol red in a sample with an absorbance at 550 nm of 0.109 *Give your answer to 3 significant figures and do not include the units (UM) in the answer. Abs orbance (550 nm) 0.160 0.340 0.120 0.100 0.060 0.040 0.020 0.000 0.0 2.0 y = 0.0145x + 0.0028 R = 0.9847 4.0 6.0 [Phenol Red] (M) 8.0 10.0 12.0

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Concept explainers

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

Question

Use the calibration curve below to calculate the concentration in uM of phenol red in a sample with an absorbance at 550 nm of 0.109
*Give your answer to 3 significant figures and do not include the units (UM) in the answer.
Absorbance (550 nm)
0.160
0.340
0.120
0.100
0.080
0.040
0.020
0.000
0.0
2.0
y = 0.0145x +0.0028
R=0.9847
4.0
6.0
[Phenol Red] (M)
8.0
10.0
12.0

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