Suppose you do an experiment in which you add to the inhibited culture (i.e., phosphoglucose isomerase is inhibited) the glucose shown below that is labeled with ¹3℃ at C3 and 14C at C4 as the sole carbon source (i.e., in this experiment the labeled glucose is the only nutrient). НО. 14C HO HT 13C OH OH -OH (a) Is ¹4CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹4CO₂? (b) Is ¹3CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹3CO₂? (c) Assume the only source of ethanol is the labeled glucose. Draw the various labeled ethanol molecules that are produced under the conditions described above. Clearly show where ¹³C and ¹4C appear in each of the different ethanol molecules. (d) What is the expected yield of ATP/glucose under these conditions (i.e., anaerobic, phosphoglucose isomerase is inhibited, and all glucose is converted to ethanol as described above). Explain your reasoning.

Suppose you do an experiment in which you add to the inhibited culture (i.e., phosphoglucose isomerase is inhibited) the glucose shown below that is labeled with ¹3℃ at C3 and 14C at C4 as the sole carbon source (i.e., in this experiment the labeled glucose is the only nutrient). НО. 14C HO HT 13C OH OH -OH (a) Is ¹4CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹4CO₂? (b) Is ¹3CO₂ produced under these conditions? If so, what percentage of the total CO₂ produced is ¹3CO₂? (c) Assume the only source of ethanol is the labeled glucose. Draw the various labeled ethanol molecules that are produced under the conditions described above. Clearly show where ¹³C and ¹4C appear in each of the different ethanol molecules. (d) What is the expected yield of ATP/glucose under these conditions (i.e., anaerobic, phosphoglucose isomerase is inhibited, and all glucose is converted to ethanol as described above). Explain your reasoning.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter27: Metabolic Integration And Organ Specialization

Section: Chapter Questions

Problem 20P: Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy...

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Question

Suppose you do an experiment in which you add to the inhibited culture (i.e.,
phosphoglucose isomerase is inhibited) the glucose shown below that is labeled with ¹³℃ at C3 and
14C at C4 as the sole carbon source (i.e., in this experiment the labeled glucose is the only nutrient).
HO.
14C
OH
НО
13C
OH
OH
(a) Is ¹4CO₂ produced under these conditions? If so, what percentage of the total CO₂
produced is ¹4CO₂?
(b) Is ¹3CO₂ produced under these conditions? If so, what percentage of the total CO₂
produced is ¹3CO₂?
(c) Assume the only source of ethanol is the labeled glucose. Draw the various labeled
ethanol molecules that are produced under the conditions described above. Clearly show
where ¹³C and ¹4C appear in each of the different ethanol molecules.
(d) What is the expected yield of ATP/glucose under these conditions (i.e., anaerobic,
phosphoglucose isomerase is inhibited, and all glucose is converted to ethanol as
described above). Explain your reasoning.

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