The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO2 production. The addition of inorganic phosphate increases this rate. 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of Pi? Which glycolytic reactions require Pi? 2. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? How much Pi is required to generate one CO2? 3. Is CO2 generation the most reliable way to measure ethanol fermentation? Why or why not?
The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO2 production. The addition of inorganic phosphate increases this rate. 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of Pi? Which glycolytic reactions require Pi? 2. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? How much Pi is required to generate one CO2? 3. Is CO2 generation the most reliable way to measure ethanol fermentation? Why or why not?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
The unicellular
The rate of fermentation is measured by CO2 production. The addition of inorganic phosphate
increases this rate.
1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the
presence of Pi? Which glycolytic reactions require Pi?
2. Several glycolytic intermediates contain phosphate. Where did these groups come from
during the reactions? How much Pi is required to generate one CO2?
3. Is CO2 generation the most reliable way to measure ethanol fermentation?
Why or why not?
Transcribed Image Text:Lactate
C/DD
Lactate dehydrogenase
NAD+
Acetyl-CoA
synthetase
NADH + H
NAD
NADH + H
Pyruvate
dehydrogenase
LTDUD
AMP
+ PP
ATP
+ CoA
NADH+H.
COA
Glucose
NAD+
CO₂
Pyruvate
COA
Glycolysis
Phospho-
transacetylase
Acetyl-CoA
Pyruvate
formate lyase
Formate
Acetate
kinase
P₁
COA
Acetyl-phosphate
ADP
ATP
NADH
Acetate
Aldehyde
dehydrogenase
NAD
Acetaldehyde
NADH + H
COA
NAD+
Alcohol
dehydrogenase
Ethanol
ADHE
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