@) so this eniyme catalytically pufet ? Explain your keasoning. , ik (b) What is Vmaz, |-4 nomol /me and enryme saturating substrate are eded?

Q: Let us assume you have cloned and expressed the catalytic unit of ATCase. You then mix and incubate…

A: For a number of disciplines, including biochemistry, pharmacology, and medicine, an understanding of…

Q: Describe which enzymes are required for lactose and tryptophan metabolism in bacteria when lactose…

A: Operons are the working unit of “deoxyribonucleic acid” that are present between the “promoter” and…

Q: Write a balanced equation for the synthesis of TMP from dUMP that is coupled to the conversion of…

A: Biomolecules are organic molecules that occur in living systems. Biomolecules include carbohydrates,…

Q: (a) What are the Vmax and Km (including units) for Tigerzyme? b) What are the Vmax and Km…

A: According to Michaelis-Menten enzyme kinetics, when the rate or velocity of an enzyme catalyzed…

Q: . Most bacterial mutants that require isoleucine for growth also require valine. Why? Which enzyme…

A: A mutation is a permanent alteration in the sequence of nitrogenous bases of a DNA molecule.

Q: What is the optimal temperature for catalase? Why do you think it becomes less effective at high or…

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Q: As described in 4C above, PLP-dependent enzymes can generate formaldehyde from serine. This compound…

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Q: Based on this information, what type of enzyme would ornithine decarboxylase be? I searched it up on…

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Q: what is the optimal pH and temperature for 6-O-α-L-ramnosyl-D-glucosidase, EC 3.2.1.168, from…

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Q: Predict the effect of mutating Asp 102 of trypsin to Asn (a) on substratebinding and (b) on…

A: Serine and cysteine proteinases form two important enzymes classes. They use a catalytic triad to…

Q: D) What are the different substrate specificities of elastase, trypsin, and chymotrypsin? And, why,…

A: Serine proteases are a group of enzymes that have a characteristic serine residue in their catalytic…

Q: Match the specificity pocket (identified by red number) to the residue that occupies it. 1 3 4 2 1.…

A: Proteases preferentially hydrolyze polypeptide substrate peptide bonds based on the amino acids…

Q: If the substrate for an enzyme catalyzed reaction contained a negative charge, which of the…

A: We are authorized to answer one question at a time since you have not mentioned which question you…

Q: 15. A substrate binds to enzyme active site. a) List the roles of serine, histidine and aspartic…

A: The enzyme has serine, histidine and aspartate in the active site.  Serine proteases are protein…

Q: Do you think that catalase should be able to attach to other substrates besides H2O2? Why or why…

A: Catalase is an enzyme that catalyzes the reaction by which hydrogen peroxide is broken down into…

Q: Predict the locations of 14C in Aspartate synthesis using the following labeled 14C succinate.

A: Given, Structure of radio label succinate: -OO14C-CH2-14CH2-14COO- Succinate is an intermediate of…

Q: Suppose a brief pulse of 14CO2 is taken up by a green plant.(a) Trace the 14C label through the…

A: The irreversible transformation of fructose-6-phosphate (F6P) and ATP into fructose-1,6-bisphosphate…

Q: Suppose a brief pulse of 14CO2 is taken up by a green plant.(a) Trace the 14C label through the…

A: In green plants carbon dioxide is fixed by an enzyme called RUBISCO. This enzyme adds CO2 to five…

Q: a) Based on the mechanism shown in Figure 2A, what type of enzyme is transpeptidase? : Lyase…

A: Note : Hi ! Thank you for the question. We are authorized to answer three subparts at a time. Since…

Q: 16. This figure from Foundations should look familiar. Which protein is shown here? catalytic triad…

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Q: The surface of E. coli ACP has a patch of Glu side chains. What can you conclude about the side…

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Q: Bovine ribonuclease folds with ΔH° = -280 kJ mol-1 and ΔS° = -0.79 kJ mol-1 K -1. Assume ΔH° and ΔS°…

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Q: Predict the eff ect on lysozyme’s activity of mutating Glu 35 to Asp and Asp 52 to Glu.

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Q: If a reaction has a ΔG°′ value of at least −30.5 kJ · mol−1, suffi -cient to drive the synthesis of…

A: The Standard Gibbs free energy change (∆G°) is the change in free energy when the substrate is…

Q: The compound triclosan inhibits bacterial enoyl-ACP reductase. Explain why triclosan is useful as a…

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Q: Approximately how much does staphylococcal nuclease (Table) decrease the activation free energy ΔG‡…

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Q: Under normal circumstances, serine residues are not very reactive. How can serine 195 be used as a…

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Q: The rate constant for the uncatalyzed reaction of two molecules of glycine ethyl ester to form…

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Q: Draw the catalytic triad of a serine protease at the first tetrahedryl intermediate stage.  Your…

A: Serine proteases cleaves peptide bonds of protein substrates. They are called serine proteases…

Q: From the complete oxidation of glucose (glucose  →  6CO2), how many total nucleotide triphosphates…

A:

Q: at what substrate concentration is v= 5.0 mM s^-1 for the enzyme catalyzed hydrolysis of trehalose?

A: 1 molecule trehalose is hydrolyzed into 2 glucose molecules by the trehalase enzyme.

Q: Define substrate level phosphorylation. How does 1,3-bisphosphglycerate relate to substrate level…

A: Introduction: Substrate-level phosphorylation  is a mechanism of ATP formation. there is  transfer…

Q: Why is ti advantageous for a cell to expend metabolic energy to polymerize gulucose molecule.?

A: Human blood possesses sugar in the form of glucose, it is being carried to all cells as a source of…

Q: Construct the mechanism of a serine-protease with a catalytic triad.

A: A serine protease is an enzyme that cleaves peptide bonds in proteins and peptides. It possesses a…

Q: 25) Although the UV280 extinction coefficient of BSA is well known, why was the BCA assay employed…

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Q: From the complete oxidation of glucose (glucose  →  6CO2), how many total nucleotide triphosphates…

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Q: Explain the structural basis for cooperative substrate binding and allosteric control in ATCase.

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Q: Which substrate is the best substrate for chymotrypsin? O N-acetylphenylalanine ethyl ester with Km…

A: Chymotrypsin is a digestive enzyme component of pancreatic juice acting in the duodenum where it…

Q: What role do low-barrier hydrogen bonds play in serine protease catalysis?

A: A low barrier hydrogen bond is a special type of hydrogen bond which can occur when the pKa of the…

Q: Please determine the template sequence used in a pyrosequencing reaction in the following graph. A…

A: Ans: The sequencing by synthesis method of DNA sequencing is referred to as Pyrosequencing.

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.