SEPARATION OF COMPONENTS BY CENTRIFUGATIONConsider the following subcellular components and their corresponding sedimentation rates.Table 3. Sedimentation rates of four subcellular components for isolation.CENTRIFUGAL FORCE (xg)SUBCELLULAR COMPONENTCDCP14896AJRA967AGC382JCKSP2378Two centrifuge machines are available in your laboratory. Centrifuge A has a rotor diameter of 14 cm anda maximum speed of 3500 rpm while centrifuge B has a rotor diameter of 18 cm and a maximum speed of14500 rpm.Construct a separation scheme of the four subcellular components considering the following parameters:The four components are separated into pellet/supernatant I and pellet/supernatant II.Only two centrifugation steps are performed; specify the centrifugal speeds (in rpm) per step. Showrelevant calculations.Both centrifuge machines must be used in the procedure.Components of each fraction are indicated.

RPM and centrifugal force: RMP is simply the rotation speed of any centrifuge machine, higher the…

Answered: SEPARATION OF COMPONENTS BY…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.
Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.
After filtering off the yeast in a difficult process, using a Celite pad to the filtrate which contains the reduced product from the ethylacetoacetate: sodium chloride solution is added. What is this process called and why is it added? IT ALT+FIN Prir Al T-FN-F10 (Mac)
what is the most efficient way to solve this dilution word problem?
When bacteria are grown under adverse conditions, i.e., in the presence of a poison such as an antibiotic, most cells grow and proliferate slowly. But it is not uncommon that the growth rate of a bacterial culture kept in the presence of the poison is restored after a few days to that observed in its absence. suggest why this may be the case.
After taking an aliquot of your cells and diluting them with Trypan Blue in a 1:1 dilution, you load the cell/Trypan mixture onto a hemocytometer to count the cells. You obtain the following counts: Quadrant Transparent cells Blue cells 1 2 3 4 LO 65 5 71 68 73 2 4 1 3 67 4) If your original cell culture (the one you took an aliquot from to count) has 13 mL of cell suspension, what is the total number of cells you have in culture? Provide your answer as the full number 2
A bacterial culture that has been pretreated with penicillin to inhibit its peptidoglycan synthesis is split and put into solutions of various concentrations. The same is done with a control culture with no prior penicillin exposure. Which condition is likely to demonstrate the biggest difference in outcomes between the control culture and the experimental culture? Placing the cultures into an isotonic solution Placing the cultures into a hypotonic solution Placing the cultures into a hypertonic solution The control and experimental cultures should show you the same results in every condition
Answer completely and correctly: A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colonymorphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?
Bacterial generation times for four different bacterial species were calculated in the media listed in Table 6.3. All media were prepared with pure distilled water and incubated aerobically in the light. Compare and contrast the growth requirements of the four bacteria listed above. Which of the media, if any, are chemically defined?| Generation Time Escherichia coli Pseudomonas Lactobacillus Nitrobacter Medium aeruginos a NaCl, NO3", MgSO4 80 Glucose 100 Glucose, NaCl, PO43- Glucose, NaCl, PO43-, MgSO4 Glucose, NaCl, PO4-, MgSO4, 56 200 43 100 28 40 8 amino acids Glucose, NaCl, PO43-, MgSO4, 25 25 80 19 amino adds
Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred
Prepare a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Draw a dilution scheme to achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.
Bacteria can increase in their number by binary fission into two daughter cells. The rate of exponential growth of a bacterial culture is expressed as generation time, how can the growth curve effect on generation time bacterial؟

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