Please written by computer source 1. We want two T-75 flasks, each with 200,000 cells in the flask that holds 20 mL of culture medium per flask. Hemocytometer counting of cells in a 1 mm sq2 area gave an average of 12 cells from a 1:9 dilution of the cell suspension.   a.) Determine the cells/mL. b.) What is the volume of your cell suspension

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1. We want two T-75 flasks, each with 200,000 cells in the flask that holds 20 mL of culture medium per flask. Hemocytometer counting of cells in a 1 mm sq2 area gave an average of 12 cells from a 1:9 dilution of the cell suspension.  

a.) Determine the cells/mL.

b.) What is the volume of your cell suspension needed to make the flasks?

c.) How you would now make the flasks as a new subculture of cells for incubation?

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