continuous disc stack centrifuge is operated at 5000 rpm for separation of tukers' yeast. At a feed rate of 60 min21, thirty of the cells are recovered. For operation at constant centrifuge speed, solids recovery is inversely proportional to the flow rate. (a) What flow rate is required to achieve 90% cell recovery if the centrifuge speed is maintained at 5000rpm thi

Q: You are examining a growth curve data from an experiment. At time 70 min, the optical density was…

A: Bacteria grow very fast if the get the proper medium for their growth and the generation time is the…

Q: Experiment of batch fermentation of E. coli in a stirred tank bioreactor was carried out.  0.5g / L,…

A: Scale  On X axis, 2cm= 0.5 hours On Y axis, 1cm= 0.02 OD

Q: Culture

A: Perhaps the most convincing long haul worldwide manageability objective isn't simply to lessen the…

Q: For the production of a secondary metabolic by Streptomyces coelicolor, a fed batch was performed.…

A: Fed-batch culture is a method of operation in biotechnological processes where one or more nutrients…

Q: What does an ‘I’ result mean in disk diffusion susceptibility test?

A: Susceptibility tests determine a microbe's vulnerability to antimicrobial drugs by exposing a…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: Graph of Absorbance vs Time(mins) 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) -Group…

A: growth rate : it is the rate at which the number of population of bacteria increases in a culture…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell fractionation is a process that involves rupturing cells, separating and suspending cellular…

Q: en gas, continuous air supply at air fl

A: The static gassing-out method was used to evaluate the impact of process factors on the volumetric…

Q: For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much…

A: Serial dilution is one of the fundamental techniques carried out in chemical and biology…

Q: Tetrahymena thermophili protozoa have a doubling time of 6.5 hrs. when grown using bacteria as a…

A: Chemostat :It is a bioreactor in which fresh medium is continuously added, while cultured liquid…

Q: As a public health engieer you have been tasked wiyh designing a plug flow reactor for the…

A: The Surface Water Treatment Rule, or SWTR for short, is an EPA regulation that establishes drinking…

Q: (b) Define concentration polarization and polarization modulus. How is polarization modulus…

A: CP is a process of accumulation of retained solutes in the membrane boundary at the feed side. It is…

Q: Before starting the freeze-drying process, the weight of the test tube, Wi which is 15.33g and test…

A: Given, Weight of the test tube is 15.33g Test tube weight with microbial cell pellets is 34.94g…

Q: A continuious, stirred-tank bioreactor with an actively growing culture of bacteria is operating at…

A: A bioreactor is a manufactured device or a system which supports biologically active environment…

Q: You have just measured the OD of your E. coli (which is in log-phase growth) and it is at 0.15. You…

A: OD stands for optical density which is the quantitative measure concentration of E. coli cells in…

Q: When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the…

A: Tiny, simple sack-like structures present in cytoplasm of cells are lysosomes. These are also known…

Q: You are planning an experiment to treat cells in culture with compound A at a final concentration of…

A: In an experiment, a control group is a group that doesn't get the treatment being tested. Its…

Q: Inoculate 250-μL overnight cell culture into 50 ml LB medium (in a 250 ml flask). Shake vigorously…

A: Cell culture requires specific media according to need of the cells. The media after inoculation is…

Q: A scientist tries to harvest bacterial cells by centrifugation; he used an RPM of 5000. If the…

A: Centrifugation is a process in which molecules are segregated out at high speed but as different…

Q: average membrane pore radius for this membrane using this information.

A: Given, Average radii=1nm Conc. of filtrate = 2.17 g/L Conc. of feed-side surface = 8.52 g/L Mass…

Q: The production of a product P from a particular gram-negative bacteria follows the Monod growth law…

A: Given that,                       rg=μmaxCsCcKs+Cs with     μmax=1h-1, Ks=0.25g/dm3,…

Q: Under certain conditions, E. coli cells grow from a concentration of 0.1 to 0.5 kg/m 3 in 1 hour.…

A: Given data:- Initial concentration of bacteria=X = 0.1 kg/m3  Final concentration of bacteria=Xt =…

Q: Gel-filtration chromatography separates molecules according to their size . Smaller molecules…

A: Gel-filtration chromatography is a type of separation technique that uses a gel as a medium through…

Q: Compute for the bacteria/ml of raw milk at dilution factor of 100, if bacterial cell counts in 10…

A: Bacteria  Sub microscopic organisms that are found almost every where on earth. They may be useful…

Q: Based on the picture write description changes in the morphology of the cultures and the presence of…

A: Plant tissue culture (PTC) refers to in vitro culturing of plant seeds, explants, plant organs,…

Q: Calculate the colony forming units (CFU) if 100  colonies grow in agar using  a 0.01-mL calibrated…

A: One colony equals to 100 col/mL in  0.01 -mL calibrated loop.

Q: Consider a batch culture of sphere-shaped bacteria in a growth medium or broth, in which the mean…

A: Batch culturing of microorganisms is an important part of industrial applications of microbiology.…

Q: 10. What is Relative Centrifugal Field (RCF, in g's) in a centrifuge with a rotor radius of 10 cm…

A:

Q: Description 1. The fractions obtained from differential centrifugation are enriched but not pure.…

A: Answer :- When dealing with complex biological samples, obtaining highly purified fractions is often…

Q: In the context of flow cytometry data: The Lactobacillus cells also show a clear increase in PerCP…

A: As stained with SYBRGreen (SG), Lactobacillus cells exhibit a noticeable increase in PerCP (red)…

Q: Solve the following problem: 34,000 bacteria/l have been measured 4 hours after inoculating a…

A: Given information Bacterial population after 4 hours of inoculation= 34,000 bacteria/l Bacterial…

Q: A surface water treatment plant operates round the clock with a flaw rate of 35 m3/min. The water…

A: Given data: Flow rate (Q)  35 m3/min Water temperature 15 degree celsius Alum dosage 25 mg/l Gt…

Q: If you need 2.5 mg/liter, 2,4-D in 1 liter of medium and the stock solution of 2,4-D contains 10…

A: The culturing of plant cells, tissue, and organs using a nutrient culture medium under a sterile…

Q: An aerobic culture is grown in a continuous bioreactor at 28C and the product of yield was found to…

A: Introduction A bacterial culture in which growth is allowed to take place in an environment with…

Q: Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time…

A: Growth rate of bacteria is the speed or rate at which the bacterial population grows per unit time…

Q: Which growth condition shown above has the largest doubling time? How would you explain the…

A: * Anaerobic condition can be seen when the uptake or lack of oxygen is greater than its production…

Q: 3) If Hybridoma cells are cultured supported in an agarose matrix in which nutrients and oxygen are…

A: A hybrid cell utilized as the purpose for the generation of antibodies in huge quantities for…

Q: Why yeast grow faster it growth medium is supplemented with erythromycin under aerobic growth…

A: Whenever any growth medium is inoculated with yeast, it is not just yeast that starts growing. It is…

Q: Question is attached

A: Note - we answer one question at a time.   Gas chromatography is a technique that is used for the…

Q: The following data were obtained during batch growth of the Saccharomyces Cerevisiae at its optimal…

A: There are billions of microorganisms that are present in our surroundings. These microorganisms keep…

Q: Calculate the mean generation time of a bacterium that increased its number from 1 x10° cells/ml to…

A:

Q: 1. Determine the inactivation rate constant (K) for a microorganism for the following treated…

A: The inactivation of microorganisms in a disinfection plant is determined by the length of time the…

Q: Explain briefly why choosing a specific standard is less important for determining the concentration…

A: A standard is a commonly accepted and agreed-upon measurement or criterion used for comparison or…

Q: What are the two appropriate control groups if flask 2: ‘Yeast + Glucose + Water’ is your…

A: Fermentation:  It is a metabolic process of carbohydrates which happens in the absence of Oxygen.…

Q: 250 uL of 1M azide was added to a 25 mL yeast solution. What was the concentration of azide in the…

A: Azide is a strong inhibitor of yeast mitochondrial respiration. It obstructs the electron transport…

Q: Determine the concentration of L. monocytogenes enumerated from the apple slice based on the…

A: Serial dilutions are done on regular basis in the microbiology laboratory to take counts of bacteria…