Select all that apply to a negative stain: O uses only 1 dye in the procedure uses multiple dyes in the procedure O involves fixing O does not involve fixing O involves a washing step does not involve a washing step uses a basic or positively charged dye which stains the bacterial cells Ouses an acidic or negatively charged dye which stains the background can show cell morphology, size, and arrangement

Select all that apply to a negative stain: O uses only 1 dye in the procedure uses multiple dyes in the procedure O involves fixing O does not involve fixing O involves a washing step does not involve a washing step uses a basic or positively charged dye which stains the bacterial cells Ouses an acidic or negatively charged dye which stains the background can show cell morphology, size, and arrangement

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

List the primary stain, secondary stain, mordant, and decolorizer for the gram stain, and write them in the order that you would use them. Make sure to label which is which.
After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.
Define dyes and describe the basic chemistry behind the processof staining.
Which of the following is an advantage of confocal microscopy over conventional fluorescence microscopy? The interaction of the laser beam with the cell surface allows the imaging of individual macromolecules O Only out-of-focus light enters the microscope lens, making the object appear illuminated against a dark background The use of electrons instead of light to image the specimen results in greatly increased resolving power O Optical sections in a single plane of focus can be visualized at different depths in the specimen The natural contrast of an unstained specimen is enhanced
What is the advantage of gram staining over simple staining
Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells
What is the principle of Grams staining based on the cell wall composition of bacterial cell.
Which two staining techniques employ a chemical (using a reagent, not a physical method) mordant? Gram stain; Acid-fast stain. O Gram stain; Capsule stain. O Capsule stain; Flagella stain. Gram stain; Flagella stain. O Simple stain; Acid-fast stain.
Name the reagents used in Gram-staining and their roles in the staining procedure
A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…
What is the identification of the unknown bacteria
which statemts pertin to Gram stain, acid-fast stain, or spore stain. please group statements with the correct term they pertain to Here are the descriptions: -does not require heat to be used in staining process -distinguishes between active metabolic cells and and dormant structures -a chemical is used as a mordant -differentiates cell with high lipid content in cell wall -uses carbol fuchsin, acid alcohol and methylene blue uses malachite green -differentitates cells based on thickness of peptidoglycan layer -result is purple and red/pink cells -important diagnostic tool in mycobacterium infections