Which of the following is an advantage ofconfocal microscopy over conventionalfluorescence microscopy?The interaction of the laser beam with thecell surface allows the imaging of individualmacromoleculesO Only out-of-focus light enters themicroscope lens, making the object appearilluminated against a dark backgroundThe use of electrons instead of light toimage the specimen results in greatlyincreased resolving powerO Optical sections in a single plane of focuscan be visualized at different depths in thespecimenThe natural contrast of an unstainedspecimen is enhanced

One significant advantage of confocal microscopy compared to conventional fluorescence microscopy is…

Answered: Which of the following is an advantage…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit-.0025 mm 4 points A) Cell 1 on 10x=8 reticular units B) Cell 2 on 40 x = 28 reticular units mm mm Which one is actually larger? Which one is probably the plant cell? Why?
FRET microscopy can be used for: a. none of the above b. electron microscopy c. centrifugation d.sensing kinase activity e. all of the above
getty Ed Res • You view this cell with the 40x objective lens and it is 7 optical units wide. Use the table below to determine the size of this cell. 1 ocular unit = Objective lens 25 um 4 x 10 μη 10 x 2.5 um 40 x 100 x 1 µm
A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…
The resolving power of the microscope reveals: a. Whether or not oil is needed to see a microorganism. b. The maximum magnification possible with a particular objective. closely spaced objects to be seen clearly. c. Two closely spaced objects to be seen clearly. d. The magnification capacity of the microscope E.the amount of clearance between slide and objective lens.
Explain briefly why the 100X objective lens of the light microscope is immersed in oil.
1. Why is it helpful that the transmission compound light microscopes we studied in lab are both parfocal and parcentered? O Why would it be harder to find a specimen on a slide starting with the 40x objective lens compared to the 4x objective lens? Support your answer with facts about the field of view and the comparison of both objective lenses. If I wanted to observe something, let's a virus that was 50 nm in size which microscope would you recommend me using? Support your answer with facts about the microscope's utility.
The microscopic preparation of the tomato pulp cells was wet mount and temporary. O wet mount and permanent. O sectioned preparation. O permanent. O all options are incorrect.
A patient comes into a hospital and has what appears to be an infection. Your microscope is broken so staining is not an option. Design an experiment to determine if the infection is caused by gram positive or gram negative bacteria.
Image A: Image B: 25 um O A. image A- scanning electron microscope, Image B- phase contrast microscope O B. Image A brightfield microscope; Image B- darkfield microscope O C.image A- brightfield scanning microscope Image B- phase contrast scanning microscope D. image A- transmission electron microscope, Image B- scanning electron microscope O E. image A- scanning electron microscrope, Image B- transmission electron microscope
which statemts pertin to Gram stain, acid-fast stain, or spore stain. please group statements with the correct term they pertain to Here are the descriptions: -does not require heat to be used in staining process -distinguishes between active metabolic cells and and dormant structures -a chemical is used as a mordant -differentiates cell with high lipid content in cell wall -uses carbol fuchsin, acid alcohol and methylene blue uses malachite green -differentitates cells based on thickness of peptidoglycan layer -result is purple and red/pink cells -important diagnostic tool in mycobacterium infections
None

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