Which of the following is an advantage ofconfocal microscopy over conventionalfluorescence microscopy?The interaction of the laser beam with thecell surface allows the imaging of individualmacromoleculesO Only out-of-focus light enters themicroscope lens, making the object appearilluminated against a dark backgroundThe use of electrons instead of light toimage the specimen results in greatlyincreased resolving powerO Optical sections in a single plane of focuscan be visualized at different depths in thespecimenThe natural contrast of an unstainedspecimen is enhanced

One significant advantage of confocal microscopy compared to conventional fluorescence microscopy is…

Answered: Which of the following is an advantage…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit-.0025 mm 4 points A) Cell 1 on 10x=8 reticular units B) Cell 2 on 40 x = 28 reticular units mm mm Which one is actually larger? Which one is probably the plant cell? Why?
FRET microscopy can be used for: a. none of the above b. electron microscopy c. centrifugation d.sensing kinase activity e. all of the above
getty Ed Res • You view this cell with the 40x objective lens and it is 7 optical units wide. Use the table below to determine the size of this cell. 1 ocular unit = Objective lens 25 um 4 x 10 μη 10 x 2.5 um 40 x 100 x 1 µm
Imagine you are using a microscope with 5x eye pieces (instead of 10x like ours). If you change from the 10x objective lens to the 40x objective lens, how does the total magnification change? Group of answer choices: -it is 5 times stronger with the 40x -it is 5 times weaker with the 40x -it is 4 times stronger with the 40x -it is 30 units weaker -it is 30 units stronger -it is 4 times weaker with the 40x
Image A: Image B: 25 um O A. image A- scanning electron microscope, Image B- phase contrast microscope O B. Image A brightfield microscope; Image B- darkfield microscope O C.image A- brightfield scanning microscope Image B- phase contrast scanning microscope D. image A- transmission electron microscope, Image B- scanning electron microscope O E. image A- scanning electron microscrope, Image B- transmission electron microscope
Pick the best form of microscopy to use in each situation, each answer used only once: You want to figure out if a bacterium can ✓ [Choose ] swim in a liquid medium You want to visualize the repeating crystalline structure of the S-layer You want to identify the basic cell wall structure of an unknown bacterium You want to watch proteins of the divisome to see how they change their location in dividing cells You want to visualize a single membrane protein complex on the surface of a cell Atomic Force Microscopy Phase-contrast microscopy Light microscopy (with staining) Fluorescence microscopy Electron microscopy [Choose] [Choose] [Choose ]
The following fluorescence microscopy shows several cells. What part of the cell is labelled by the fluorescence here? b The Cytosol The Endoplasmic Reticulum The nucleolus The nucleus The actin cytoskeleton
What is the purpose of using microscopes? If you need to move the microscope, describe how you should carry it. What three things change as you increase magnification? On the compound microscope, what power must be in place when you first find an image in themicroscope? On the compound microscope, which adjustment knob is used for bringing the specimen intofocus? On the compound microscope, which adjustment knob is used for sharpening the image of thespecimen after it is focused? On the compound microscope, which adjustment knob should you use on the high power? On the compound microscope, how do you change the objective lenses? What should be included when sketching or drawing what is seen through the microscope How do you properly use a microscope?
do both or don't attempt

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