Relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation. a)At what substrate concentration would an enzyme with a kcatof 30.0 s-1and a Kmof 0.0050 M operate at one-quarter of its maximum rate? b)Determine the fraction of Vmax that would be obtained at the following substrate concentrations [S]:1/2Km,2Km,and10Km. c)An enzyme that catalyzes

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Relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation.

a)At what substrate concentration would an enzyme with a kcatof 30.0 s-1and a Kmof 0.0050 M operate at one-quarter of its maximum rate?

b)Determine the fraction of Vmax that would be obtained at the following substrate concentrations [S]:1/2Km,2Km,and10Km.

c)An enzyme that catalyzes the reaction X ßàY is isolated from two bacterial species. The enzymes have the same Vmax, but different Km values for the substrate X. Enzyme A has a Km of 2.0 μM, while enzyme B has a Km of 0.5 μM. The plot below shows the kinetics of reactions carried out with the same concentration of each enzyme and with [X]=1 μM. Which curve corresponds to which enzyme?

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