7. (a) Consider the following hypothetical metabolic pathway: (d) Briefly summarize the regulatory advantage(s) of a substrate cycle in a pathway. A B C D Under intracellular conditions, the activity of enzyme X is 100 pmol/10° cells/s. Calculate the effect on the metabolic flux rate (pmol/10 cells/s) of B →Cof the following treatments. Calculate as % change (increase or decrease). Treatment % Change in flux Inhibitor that reduces activity of X by 10% Activator that increases activity of X by 10% (b) Now consider a substrate cycle operating with enzymes X and Y in the same hypothetical metabolic pathway: A B Y Under intracellular conditions, the activity of enzyme X is 100 pmol/106 cells/s, and that of enzyme Y is 80 pmol/10° cells/s. What are the direc- tion and rate (pmol/106 cells/s) of metabolic flux between B and C? (c) Calculate the effect on the direction and metabolic flux rate of the fol- lowing treatments. Calculate as % change (increase or decrease). Direction of flux (В — Сor C —> В) Treatment % Change in flux Inhibitor that reduces activity of X by 10% Activator that increases activity of X by 10% Doubling the activity of enzyme Y

7. (a) Consider the following hypothetical metabolic pathway: (d) Briefly summarize the regulatory advantage(s) of a substrate cycle in a pathway. A B C D Under intracellular conditions, the activity of enzyme X is 100 pmol/10° cells/s. Calculate the effect on the metabolic flux rate (pmol/10 cells/s) of B →Cof the following treatments. Calculate as % change (increase or decrease). Treatment % Change in flux Inhibitor that reduces activity of X by 10% Activator that increases activity of X by 10% (b) Now consider a substrate cycle operating with enzymes X and Y in the same hypothetical metabolic pathway: A B Y Under intracellular conditions, the activity of enzyme X is 100 pmol/106 cells/s, and that of enzyme Y is 80 pmol/10° cells/s. What are the direc- tion and rate (pmol/106 cells/s) of metabolic flux between B and C? (c) Calculate the effect on the direction and metabolic flux rate of the fol- lowing treatments. Calculate as % change (increase or decrease). Direction of flux (В — Сor C —> В) Treatment % Change in flux Inhibitor that reduces activity of X by 10% Activator that increases activity of X by 10% Doubling the activity of enzyme Y

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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