Question with regards to SDS-PAG You are working with a unique protein that has no basic amino acids and contains a lot of acidic amino acids. You run a SDS-PAGE gel, you stain and destain it. You get nice bands for your ladder but no bands for your sample proteins. After doing an immunoblot specific for your protein (using the SDS-PAGE gel), you do get a band identifying your protein. You know that your concentration of the protein is sufficient to be visualized by staining it. The immunoblot clearly identifies that your protein is in your gel, why can you not see it after staining and destaining the gel?
Question with regards to SDS-PAG You are working with a unique protein that has no basic amino acids and contains a lot of acidic amino acids. You run a SDS-PAGE gel, you stain and destain it. You get nice bands for your ladder but no bands for your sample proteins. After doing an immunoblot specific for your protein (using the SDS-PAGE gel), you do get a band identifying your protein. You know that your concentration of the protein is sufficient to be visualized by staining it. The immunoblot clearly identifies that your protein is in your gel, why can you not see it after staining and destaining the gel?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question with regards to SDS-PAG
You are working with a unique protein that has no basic amino acids and contains a lot of acidic amino acids. You run a SDS-PAGE gel, you stain and destain it. You get nice bands for your ladder but no bands for your sample proteins. After doing an immunoblot specific for your protein (using the SDS-PAGE gel), you do get a band identifying your protein. You know that your concentration of the protein is sufficient to be visualized by staining it. The immunoblot clearly identifies that your protein is in your gel, why can you not see it after staining and destaining the gel?
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