Question 4 About endonucleases: A Restriction endonucleases always cut double-stranded DNA and are endo- deoxyribonucleases. B Once the DNA sequence is recognized, they cleave the bond between the desoxyri- bose and the nitrogenous base. |C they are proteins that are necessary for the replication of DNA. D they act on single-stranded DNAS, RNAS, and double-stranded DNAS. E they are enzymes whose substrate is a double-stranded sequence of nucleic acids, DNA or RNA, or hybrid DNA-RNA.

Question 4 About endonucleases: A Restriction endonucleases always cut double-stranded DNA and are endo- deoxyribonucleases. B Once the DNA sequence is recognized, they cleave the bond between the desoxyri- bose and the nitrogenous base. |C they are proteins that are necessary for the replication of DNA. D they act on single-stranded DNAS, RNAS, and double-stranded DNAS. E they are enzymes whose substrate is a double-stranded sequence of nucleic acids, DNA or RNA, or hybrid DNA-RNA.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

QUESTION 3 What type of chemical bonds hold together a DNA double helix? weak hydrogen bonds between nitrogenous bases O weak hydrogen bonds between nitrogenous bases O strong covalent bonds between phosphates O covalent bonds between deoxyribose and nitrogenous bases O hydrogen bonds between hydroxyl groups
QUESTION 6 Which of the following is the correct term for the fact that DNA bends around and forms a shape that resembles a spiral staircase? O A. semi-conservative O B. antiparallel O C. condensed chromatin O D. double helix
QUESTION 3 which of the following is true for methyl directed repair O A. methyl directed repair cannot distinguish the template strand from the newly replicated strand. O B. methyl directed repair changes both the template strand and the newly replicated strand. O C. methyl directed repair corrects the DNA strand that is methylated. O D. methyl directed repair corrects the mismatch by changing the newly replicated strand. O E. methyl directed repair corrects the mismatch by changing the template strand.
question 6
QUESTION 4: Why does a PCR reaction require a primer? Would you expect this primer to be composed of DNA or RNA? Explain your reasoning.
Question 10 Produce the complimentary DNA strand that would be matched with the provided strand T--A C-- G C-- G A -- A -- C-- G-- T-- T-- A -- C-- G -- G--
QUESTION 5 If DNA had 5 different bąses, a restriction enzyme with a 3-base recognition sequence would cut DNA approximately every O A 125 bp. O B. 256 bp. O C. 425 bp. O D. 625 bp. O E. 3125 bp.
QUESTION 3 Is it possible to dissolve DNA in water? a. Only during cell division b. No, DNA is soluble only in lipids c. Yes, DNA is polar due to the presence of sugars and charged phosphate groups d. In ancient times yes, however, DNA became non-water soluble very recently. QUESTION 4 What are the solutions to extract DNA in your experiment? a. hydrochloric acid; aniline b. water, oil c. Biuret; water d. water; ethanol
Question 9 What is the reason that the Taq polymerase is currently used? (A) the Taq polymerase is one of the most rapid DNA polymerase enzymes known and is therefore extremely useful for PCR (B) none of the given is correct Cthe Taq polymerase is the only DNA polymerase that will allow production of 2n amounts of DNA (where n-number of cycles) (D) the DNA melting step at 95°C would cause denaturation of most DNA polymerases from other organisms
Question 5 Review DNA sequencing and cloning tools. Which of these is not used to make a recombinant DNA? O restriction enzymes to create sticky ends of a plasmid O fragment from a different DNA cut by the same restriction enzyme O DNA ligase seals the recombinant DNA O denaturation
Question 1 of 10 Question 1. You isolate a mouse Tau-gene-containing DNA fragment from the chicken and hybridize it to the freshly-made and isolated hnRNA (primary transcript) from the nucleus of the mouse cells transcribed from the Tau gene (immediately after it was produced), allowing no time for processing of the hnRNA. Describe what you see when you look at the DNA/RNA hybrid molecule under the electron microscope.
QUESTION 6 These correct “overwinding” ahead of the replication forks by breaking, swiveling, and rejoining DNA strands. Helicases Single-strand binding proteins Topoisomerase Telomerase