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Answer the above question for CutVI if the starting DNA were linear instead of circular.

Answer the above question for CutVI if the starting DNA were linear instead of circular.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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st your set of assigned enzymes and the expected fragment sizes here. Base Pairs 1. Using the gel picture to the left, show where your digestion of the paper 1,000 - 900 - cutting model showing cut fragments would appear. The linear uncut DNA is 640 base pairs in length. 800 - 700 - 600 - 500 - 400 - 2. Explain why the restriction endonucleases BglII (A^GATCT) and Xbal (T^CTAGA) will not recognize and cut the restriction site of the other. 300 - 200 - 100 - 3. What are the last three of six nucleotides (bases) of the palindromic site for 50 - the enzyme Ndel if the first three are C AT ? 4. Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA? 5. If the hypothetical restriction endonuclease CutVI were to recognize and cleave a circular piece of DNA at 6 different sites, how many fragments of DNA would result? 6. Answer the above question for CutVI if the starting DNA were linear instead of circular. 7. If the electrical leads were reversed by mistake (red connected to black), what would be the result? The gel would run backwards, with DNA moving the other direction. The DNA would separate as usual, only now the larger pieces would travel farther and smaller pieces shorter distances. The DNA would not move at all. A. В. С. 8. Explain your answer choice for the previous question in rational terms. DNA ladder Paper DNA digest
Transcribed Image Text:st your set of assigned enzymes and the expected fragment sizes here. Base Pairs 1. Using the gel picture to the left, show where your digestion of the paper 1,000 - 900 - cutting model showing cut fragments would appear. The linear uncut DNA is 640 base pairs in length. 800 - 700 - 600 - 500 - 400 - 2. Explain why the restriction endonucleases BglII (A^GATCT) and Xbal (T^CTAGA) will not recognize and cut the restriction site of the other. 300 - 200 - 100 - 3. What are the last three of six nucleotides (bases) of the palindromic site for 50 - the enzyme Ndel if the first three are C AT ? 4. Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA? 5. If the hypothetical restriction endonuclease CutVI were to recognize and cleave a circular piece of DNA at 6 different sites, how many fragments of DNA would result? 6. Answer the above question for CutVI if the starting DNA were linear instead of circular. 7. If the electrical leads were reversed by mistake (red connected to black), what would be the result? The gel would run backwards, with DNA moving the other direction. The DNA would separate as usual, only now the larger pieces would travel farther and smaller pieces shorter distances. The DNA would not move at all. A. В. С. 8. Explain your answer choice for the previous question in rational terms. DNA ladder Paper DNA digest
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