**Question 29**In samples of DNA, isolated from two unidentified species of bacteria X and Y, adenine makes up 32% in X and 23% in Y of the total bases.**Part A**Write below the percent relative proportions of adenine, guanine, thymine, and cytosine you would expect to find in the two DNA samples.For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).---### Toolbar Explanation:The toolbar contains standard text editing tools such as:- Bold (B)- Italic (I)- Underline (U)- Strikethrough (S)In this section, users can adjust:- Paragraph alignment- Font style (e.g., Arial)- Font size (e.g., 10pt)- List formatting options- Line spacing - Text color and highlight- Additional tools for inserting images or links. This setup is typical for an environment where users are expected to compose and format text directly into the platform.

According to Chargaff's rule that the amount of purine should be equal to pyrimidine and also that…

Answered: QUESTION 29 In samples of DNA, isolated…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 10 Select all of the true statements regarding the molecule below: NH, -P-O NH G OH NH, О-Р-О- H,N N- A ОН -P-O `NH U ОН 0-P-O- N' ОН ОН This is RNA This is DNA This chain is a polymer. The backbone of these nucleotides is a ribose sugar This nucleic acid is the hereditary material for humans. This nucleic acid is the hereditary material for coronaviruses. This type of nucleic acid is never found in healthy human cells. This nucleic acid contains the nucleobase thymine.
Question #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…
QUESTION 5 If you ran the amplicon from the previous question (so it's about 3.7k basepairs long), on a gel, what would you want your gel to look like after you stain it with ethidium bromide and look at it under UV, assuming you ran it next to a 1kb DNA ladder? O A. A lot of lighter bands below the 4kb band O B. A very bright band at the bottom of the gel, and a lighter band in the middle of the gel. O C.A bright smear O D. one bright band that lines up between the 3kb and 4kb ladder band. Should be above the 3kb band (closer to the wells). O E. one bright band that lines up below the 3kb ladder band (further from the wells)
QUESTION 13 The two strands of a DNA molecule are held together by hydrogen bonds between the O phosphate groups on each strand nitrogenous bases on each strand bases and the phosphate-sugar backbone O carbon atoms in the sugars
question 6
Question 10 Produce the complimentary DNA strand that would be matched with the provided strand T--A C-- G C-- G A -- A -- C-- G-- T-- T-- A -- C-- G -- G--
Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' ? b) Assuming that the % GC is = 60%. O (2/10)4 (3/10)² O (3/10)4 (2/10)² O (1/6)4 O (1/4)6 O (6/10)4 (4/10)2
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
QUESTION 7 For each statement below, state whether it is true of DNA, RNA, Both DNA and RNA, or Neither DNA nor RNA using the letters indicated below: a. DNA b. RNA c. Both DNA and RNA d. Neither DNA nor RNA 1. Can contain the nucleotide Uracil. 2. The nucleotides are connected via phosphodister linkages. 3. Can contain the nucleotide Adenosine. 4. Has a secondary structure that is a double helix. 5. Is a polymer that is composed of a series of amino acids.
QUESTION 26 In a double-stranded DNA molecule, how are the sequences of each strand related to each other? A The sequences are identical. B The sequences are identical except that one strand has a U where the other strand has a T. C The sequences are the reverse compliment of each other and use the nucleotides, A, G, U, and C only. D The sequences are the reverse complement of each other.
Question 1: Look at the following normal and mutant DNA sequences. Normal Sequence (5'-3'): ATG AAC GTT ATC GCA Mutated Sequence (5'-3'): ATG AAT GTC ATC GCA a) What type of mutation has occurred (be specific)? b) Fill in the table for the normal and mutated sequences. Starting with the given 5'-3' sequence, input the complementary DNA, transcribed RNA from the 3'-5' DNA and translated polypeptide sequence for both. Hint: use the codon table! Normal sequence Mutant sequence DNA 5'-3' (given) DNA 3'-5' RNA Polypeptide (use 3 letter codes) c) Based on parts A and B above, what is the ultimate effect this mutation has had on the polypeptide? (1 sentence summary)
Question 17 Nucleotides contain all of the following except O a 5-carbon sugar. O histones. O a phosphate group. O a nitrogen base. otx PRINECOMA3_..pptx MacBook Air F2 F3 F4 F5 F6 F7 F8 @ 2$ & * 3 8. W E T Y S D H J V M. * 00 B

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