QUESTION 2 How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines). QUESTION 3 ELISA technique was used to assay all three pathways of complement. Which molecule will be adsorbed to the ELISA plate in each of the pathways. QUESTION 4 How can radial immunodiffusion be used to demonstrate complement function assay? (Answer should not be more than two lines)
QUESTION 2
How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines).
QUESTION 3
ELISA technique was used to assay all three pathways of complement. Which molecule will be adsorbed to the ELISA plate in each of the pathways.
QUESTION 4
How can radial immunodiffusion be used to demonstrate complement function assay? (Answer should not be more than two lines)
QUESTION 5
A 3-year-old child has a history of serious infections and is currently hospitalized with meningitis. The doctor suspects that he may have a complement deficiency and orders testing. A buffer that chelates calcium was added to specimen. There was a decrease radial haemolysis on agarose plate a) Which complement pathway accounted for the result obtained and how will the result in terms of hemolysis at 50 %? b) What is hydrodynamic focusing and why is it an important feature of an instrument.
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