QUESTION 19 In using a Bradford reagent to measure protein concentrations, a protein sample is diluted 100x then diluted 100x again. The concentration of the diluted sample is found to be 0.605 mg/ml. How much the undiluted sample and buffer in volume (microliter, ul) are needed to create a 1 ml solution containing the protein at 2 mg/ml concentration? O 2 ul protein| 998 ul buffer 49 ul protein | 951 ul buffer O 40 ul protein | 960 ul buffer O 20 ul protein | 980 ul buffer

QUESTION 19 In using a Bradford reagent to measure protein concentrations, a protein sample is diluted 100x then diluted 100x again. The concentration of the diluted sample is found to be 0.605 mg/ml. How much the undiluted sample and buffer in volume (microliter, ul) are needed to create a 1 ml solution containing the protein at 2 mg/ml concentration? O 2 ul protein| 998 ul buffer 49 ul protein | 951 ul buffer O 40 ul protein | 960 ul buffer O 20 ul protein | 980 ul buffer

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 6 If the 3 proteins in the blue sample had MW's of [1] 87,000 Daltons, [2] 30,000 Daltons, [3] 15,000 Daltons; what is the MW of the protein in the 2nd band (counting from the origin) of the green sample? O O O O a. about 15,000 daltons b. about 87,000 daltons c. more than 30,000 daltons but less than 87,000 daltons d. about 30,000 daltons Cellular and Molecular Biology (6657
Question 40 Protein synthesis in bacterial cells usually starts with a: (A) methionine residue. B) formylmethionine residue. C) alanine residue. (D) phenylalanine residue.
QUESTION 6 Two chambers, X and Y, are separated by a semi-permeable membrane that allows water and urea, but not glucose, to pass through. At the start, chamber X contains a solution of urea in wate and chamber Y contains a solution of glucose in water. The molarity (measure of concentration) of urea in chamber X is the same number as the molarity of glucose in chamber Y. Which of the following will occur after sufficient time? There will be overall diffusion of glucose from X to Y until glucose concentration in Y exceeds glucose concentration in X. There will be overall diffusion of glucose from X to Y until glucose concentration is equal in both chambers. There will be overall diffusion of glucose from Y to X until glucose concentration in X exceeds glucose concentration in Y. There will be overall diffusion of glucose from Y to X until glucose concentration is equal in both chambers. Glucose will not diffuse across the membrane.
Question 20
Question 14 Which of the following DOES NOT define a gel? O a.it is in a semi-solid state O b.it is connected at the point known as junction zones Oc.it is a 3-D network of interconnected molecules joined by covalent interactions d. it entraps liquid in the spaces between junction points
QUESTION 5 This amino acid has a pK1 = 2.36 and a pK2 = 9.68. Its pH/ is Оa. 7,32 Ob.6.00 Oc 12.04 Od.6.02 QUESTION 6 Cellulose is indigestible to most animals because: Oa. Cellulose has no food value and therefore cannot be digested O b. Animals lack the enzymes required to hydrolyze the a links of the cellulose O. Cellulose is not soluble in water O d. Animals lack the enzymes required to hydrolyze the B links of the cellulose
QUESTION 1 The full name of this amino acid is The 3-letter abbreviation is Please also note the following: • The non-ionized structure is shown • Spelling matters • For the acidic amino acids use the full name, e.g. "so-and-so acid" • Case does not matter • Do not include L- or D- in the name НООС- СH - NH2 CH3
QUESTION 2 You obtained the following raw data when setting up a Bradford standard curve: Absorbancy BSA (mg/ml) 595nm 0.225 0.310 2 0.420 0.510 4 0.610 0.720 0.810 0.915 0.950 9 0.980 10 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.583 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
Question 30 Match the following methods of Analysis: ✓ Lipid anchor is myristic acid and linked to a glycine ✓ Sulfur-containing amino acids are linked to lipid anchor ✓ Sulfurs of cysteine are linked to the isoprene groups ✓ Oligosaccharides are linked to lipid anchors ✓ Lipid that mostly present in the outer leaflet of the membrane ✓ Lipid that is responsible for the rigidity of the cell membrane ✓ Lipid that is only present in the nuclear and mitochondrial membrane ✓ These lipids are highly present in the inner leaflet of the membrane These lipids in the membrane recognizes apoptotic cells These lipids are involved in the production IP3 secondary messengers A. Cholesterol B. Phosphatidylcholine C. Thioester-linked and acyl anchor D. Phosphatidylinositol E. Glycosyl phosphatidylinositol anchor F. Phosphatidylserine G. Thioether-linked prenyl anchor H. Cardioliptin 1. Phosphatidylethanolamine J. Amide-linked myristoyl anchor
QUESTION 2 The following carbohydrate tests have been performed on a sample containing an unknown sugar. The results obtained are indicated as a positive (+) or negative (-) reaction for each of the tests performed. Fehling Barfoed Bial lodine Seliwanoff Benedict + Unknown Sugar Based on the carbohydrate test results obtained for unknown sugar, characterize the sugar by completing the table that follows: Characteristic Unknown sugar Answer with: Yes or No Monosaccharide Disaccharide Polysaccharide Reducing Pentose Aldose
Question 26
QUESTION 32 Aquaporins are proteins that control the passage of water molecules across a cell membrane. The protein forms a pore, or opening, in the membrane. You isolate what you think are two different molecules of aquaporin and determine that one of the proteins has a larger pore diameter than the second. Which of the following do you conclude? These two forms of aquaporin will have identical sequences of amino acids. These two forms of aquaporin will have different sequences of amino acids. You will have to sequence the proteins to compare their primary structure, because it should have no effect on pore diameter. These two forms of aquaporin have identical primary structure but differ in their tertiary structure.