Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL-1 beef extract, 3gL1 yeast extract and 5 gl1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. I a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100µg/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.
Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL-1 beef extract, 3gL1 yeast extract and 5 gl1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. I a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100µg/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.7: The Metric System
Problem 1P
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