ChemistryPlease estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3 2. AldolaseAldolase is a 157 kD enzyme that catalyzes the following reaction:AldolaseOHHOOHD-Fructose-1,6-diphosphate2.551025501[s] (uM) Vo (uM/min)13.98.115.322.028.033.0rofalanDihydroxyacetone phosphateD-Glyceraldehyde-3-phosphateIn an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detectedby modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). Theenzyme concentration for the assay is 150 pM, and the following data is obtained:OHH₂NNH₂OH

Estimation of Km and Vmax can be done from the Michaelis-Menten equation. The values of Km and Vmax…

Answered: Please estimate the Km and Vmax and…

Please estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: A pipet is used to transfer 3.00 mL of a 2.00 M stock solution in flask “S” to a 25.00-mL…

A: From the statement, it is given that Molarity of stock solution, M1= 2.00 M Let, Molarity of…

Q: Consider the following reaction (with a small amount of sulfuric acid added as a catalyst): Acetic…

A: Acetic acid(l) + Isopentyl alcohol(l) ⇔Isopentyl acetate(l) + water(l) Density of isopentyl alcohol…

Q: 2 When 50 cm' of hydrochloric acid of concentration 2.0 mol dm3 is added to 50 cm' of sodium…

Q: A pipet is used to transfer 6.00 mLmL of a 3.75 MM stock solution in flask “S” to a 25.00-mL…

A: The number of moles of compound in 25.00 mL flask “A” is; 1000 mL=1 Ln=3.75 M×6.00 mL1000…

Q: The organic base, tris-(hydroxymethyl)aminomethane (or simply TRIS or THAM) is an excellent primary…

A: Amount of the sample = 0.2486 gMolar mass of TRIS = 121.14 g/molVolume of the hydrochloric acid…

Q: Come up with a dilution scheme starting from 1000 μg/mL Mn stock solution so that the concentration…

A: Dilution scheme starting from 1000 μg/mL Mn stock solution so that the concentration of permanganate…

Q: What are the units of KM? Os molar 1/s s/molar 1/molar molar/s 0 0 0 0 0 О

A: Michaelis-Menten equation: v=Vmax[S]KM+[S] Here, v- velocity of the reaction.v=Vmax[S]KM+[S]Vmax-…

Q: 55. Volume of Titram (ml) 24.70 24.80 24.90 25.00 25.10 25.20 25.30 Potential (mV) (A) 24.96 mL (B)…

A: Given is a table of potential vs volume.To find the volume of the titrant needed to reach the…

Q: The following data give the constant dissociation K (for acetic acid at various temperatures) T(°C)…

A: The given values of the temperature and the corresponding dissociation constant values are shown in…

Q: 3. If 30.65 mL of Ca(OH), solution is needed to react with 0.240 g of primary standard H,C,0, •2H,O,…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: M Inbox (21,528) - sarat X b Answered: The enthal X ← → C app.101edu.co STARTING AMOUNT +…

Q: Stock iron(II) solution (200Ug mL-1 Fe) ferrous ammonium sulfate hexahydrate mass= 0.1437g, transfer…

A: Given mass of ferrous ammonium sulfate hexahydrate = 0.1437g, Molar mass = 392.13 g/mol Total…

Q: 5R) The net ionic equation for formation of an aqueous solution of Nil2 accompanied by evolution of…

A: Steps for writing the Net Ionic Equation : 1. Write the Balanced Molecular equation. 2.…

Q: A 20.330 g sample of aqueous waste leaving a fertilizer manufacturer contains ammonia. The sample is…

A: Titration is a volumetric analysis in which the unknown concentration of the sample is measured by…

Q: B. DETERMINATION Molarity of nitric acid, M Volume of nitric acid, mL Molarity of sodium hydroxide,…

A: Nitric acid, HNO3 is a monoprotic strong acid. Sodium hydroxide, NaOH is a monoacidic strong base.…

Q: Craw McGraw-Hill Education Campus Saved Ex. 1b - Mass/volume percent concentration - calculate using…

A: Volume of solution = 0.350 L Mass of KCl = 13.0 g We have to find m/v percent of solution.

Q: Sample 1 2 3 4 Sample Volume, mL 50.00 49.50 25.00 50.00 Ba(OH)2 Volume, mL 43.17 42.68 21.47 43.33…

A: In order to find the mean w/v percentage of acetic acid in the sample. we have to find the w/v…

Q: Solutions A and B are combined in a test tube, producing the following reaction: A + B-→ C + D Which…

Q: How to prepare the solutions listed below Solution 1) 25 mL 7 M sulfuric acid and the respective…

A: Molarity (M) is the number of moles of solute present in per liter of the solution. Its unit is…

Q: I have a stock solution of a drug that has a molarity of 10 millimolars. The desired working drug…

A: Given, I have a stock solution of a drug that has a molarity of 10 millimolars. The desired working…

Q: Pentane (C5H12) is a liquid with a density of 0.626 g/mL near 25°C. Use the equation below to find…

A: A liquid with low density has different mass in air and vacuum , this is due to the bouyancy of air.…

Q: A solution of Ca(OH)2 (FM = 74.09) was used in determining the equivalent weight of an unknown acid.…

A: Given data contains, Mass of KHP is 0.1235g. Volume of KHP is 7.45mL. Mass of unknown acid is…

Q: How many J are there in 30.0 kcal? (1 cal = 4.18 J) %3D

A: Unit conversion can be used to convert one unit into another unit with the help of conversion factor…

Q: dN F(V)dV = M 47(- 2TRT MV? ?v? cxp( 2RT N

A: Given,F(V)dV=dNN=4π(M2πRT)3/2V2exp(MV22RT)dV (i)we need to find most probable velocity…

Q: Wt of precipitate (Silver chloride) MW of silver nitrate MW of silver chloride Meg silver nitrate…

Q: A 39.4 g sample of halothane is 0.200 moles. At STP, what is the concentration (% v/v) of 39.4 g of…

A: Given: Mass of halothane = 39.4 g. Moles of halothane = 0.200 mol. Volume of container = 224 L And…

Q: STARTING AMOUNT X What quantity in moles of NH gas forms when 32.4 liters of H reacts at STP…

A: Given - Volume of one mole ideal gas=22.7L Volume of H2=32.4L Moles of NH3=?

Q: Penicillin is hydrolyzed and thereby rendered inactive by beta-lactamase, an enzyme present in some…

Q: A mixer containing 100 gal of Na,SO, solution (4 Ibs of Na2S04 per gal) is attached to a reservoir…

Q: The gravimetric factor used to express CoCBr6·H20 in a sample that is finally weighed as PbClBr is…

A: Gravimetric factor

Q: Calculate the normality of 5.627 g/L K₂Cr₂O₇ (294.19) in the reaction: Cr₂O₇²⁻ + 14H⁺ + 6e⁻ ⟶ 2Cr³⁺…

A: Normality is another way of expexpressing the concentration of solution

Q: How many mL of 0.100 M NaCl would be required to make a 0.0150 M solution of NaCl when diluted to…

A: As we know , Moles the solute remains same on dilution but volume of the solution increases.…

Q: The calcium (AW= 40.08 g/mol) from a sample of limestone weighing 607.4 mg was precipitated as…

A: From the question, The mass of the limestone sample is given = 607.4 mg The given limestone sample…

Q: How many calories are in 5401 J? (1 cal = 4.18 J)

A: The answer to the the following question is given as:

Q: A solution of Ca(OH)2 (FM = 74.09) was used in determining the equivalent weight of an unknown acid.…

A: The number of moles of KHP can be determined as follows: Number of moles of KHP = Given mass of…

Q: Q1) what is The concentration of Hg in PPm in the solution in equilibrium with Hg2(CN)2 ? PKSP (…

Q: A 4.0 molar sulphuric acid solution (SG = 1.2) is prepared by mixing two aqueous sulphuric acid…

A: A desired sulphuric acid solution is prepared by mixing two aqueous solutions of sulphuric acid.

Q: .. We want to determine the purity of alum sample bought in a local pharmacy in Miagao. A Chem 28…

A: A numerical problem based on quantitative analysis, which is to be accomplished.

Q: Aluminum sulfate can be made by the following reaction: 2AlCl3(aq)+3…

A: As you not specified so I am giving answer of first question First convert mass into mole by diving…

Q: If there was a leak in the syringe and gas was escaping as the syringe plunger was being compressed.…

A: Step 1:The ideal gas equation would bePV=nRTWhere Pis the pressureV is the volumeR is the universal…

Q: A tire pressure gauge reads 33.0 pounds per square inch How many kPa is this? Remember that multiple…

A: Pressure gauge reading = 33.0 psi (pounds per square inch)

Q: Calcium Chloride solution comes as a 10% solution in a 10 ml vial. How many ml of this solution are…

A: A quantity can be represented using multiple units. By analyzing the relationship between the two…

Q: A chemist mixes 26. g of chloroform with 23. g of acetone and 32. g of acetyl bromide. Calculate the…

Q: A STOCK SOLUTION containing 0.1581 g/L K2CrO4 was prepared. In order to make the CALIBRATION…

A: To calculate the ppm and molarity of K2CrO4 in the calibration standard, we first need to calculate…

Q: Toxic Cr(VI) can be precipitated from an aqueous solution by bubbling SO2 through the solution. How…

A: The amount of sulfur dioxide has to be calculated

Q: The enthalpy of solution (AH) of KOH is -57.6 kJ/mol. If 1.56 g KOH is dissolved in enough water to…

Q: Determine the percentage error and the concentration of the prepared NaOH given the following data:…

A: To determine the percentage error and the concentration of prepared NaOH

Q: A pipet is used to transfer 5.00 mL of a 1.25 M stock solution in flask “S” to a 25.00-mL volumetric…

A: In the given problem, the molarity of the serially diluted solution can be calculated by using the…

Question

Chemistry

Please estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3

2. Aldolase
Aldolase is a 157 kD enzyme that catalyzes the following reaction:
Aldolase
OH
HO
OH
D-Fructose-1,6-diphosphate
2.5
5
10
25
50
1
[s] (uM) Vo (uM/min)
1
3.9
8.1
15.3
22.0
28.0
33.0
rofalan
Dihydroxyacetone phosphate
D-Glyceraldehyde-3-phosphate
In an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detected
by modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). The
enzyme concentration for the assay is 150 pM, and the following data is obtained:
OH
H₂NNH₂
OH

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step by step

Solved in 4 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Similar questions

If a cylindrical tank holds 100,000 gallons of water, which can be drained from the bottom of the tank in an hour, then Torricelli's Law gives the volume V of water remaining in the tank after t minutes as 1 V (t) = 100, 000 (1- 60 for 0
An HPLC method was developed for the separation and determination of ibuprofen in blood samples. A 3.00 mL blood sample dissolved in 70.00 mL solvent was prepared and 10.0 mL was injected into the HPLC. Standard ibuprofen solutions were also run.Calculate the ibuprofen present in the 3.00 mL blood sample The following data were obtained. Table po itoo: Ibuprofen concentration, mg/ml0.50 1.0 2.0 3.0 Blood sample Peak area (Right column)5.29.617.419.911.4
00 P and Hei 3. Considering the techniques and instrumentation used to perform these experiments, identify 2 potential sources of error if you were to repeat this experiment in the "real world". Human error is not an acceptable answer. Discuss at least two possible sources of error that could contribute to your percent error. 4. A student is performing Reaction 2 and does not completely dissolve the NaOH pellets before acquiring the final temperature. How will this affect his value for AH, and his percent error calculations?
Standardization of NaOH solution using KHP *MW of KHP = 204.22 g/mol Calculate for the concentration in Molarity (M) of NaOH supplied in the burette. MASS OF KHP (g) VOLUME OF KHP Solution (mL) 250 2.725 1 2 3 Using a pipette, 50 mL aliquot was taken from the prepared solution to titrate with NaOH. TRIAL V Analyte (mL) Final Reading of the Titrant (mL) 25.1 25.3 25.2 50 50 50 Initial Reading of the Titrant (mL) 50 50 MOLARITY KHP Solution (M) 3 50 AVERAGE Volume of Titrant used (mL) 1 1 1 MOLARITY NaOH (M) 4 4 4 2
VIII. A primary standard of HCI is sodium carbonate, Na2CO3. A measured mass of solid Na2CO3, mNa2CO3' in grams, is dissolved in water and then titrated with HCI solution using methyl 2 NaCl + CO2 + H2O. orange indicator. The reaction is: Na2CO3 + 2 HCI- Complete the following formulas using the context described above: MHCI × VHCI in mL at the methyl orange endpoint = mNa2CO3 × ( )= mol of Na2CO3 mol of Na2CO3 X (— ( - - )= mol of HCl
A pipet is used to transfer 5.00 mL of a 1.25 M stock solution in flask “S” to a 25.00-mL volumetric flask “B,” which is then diluted with DI H2O to the calibration mark. The solution is thoroughly mixed. Next, 3.00 mL of the solution in volumetric flask “A” is transferred by pipet to a 50.00-mL volumetric flask “B” and then diluted with DI H2O to the calibration mark. Calculate the molarity of the solution in volumetric flask “B.”
CrO42- + HSnO2- à CrO2- + HSnO3- When this reaction is balanced for basic conditions, the coefficients for CrO42- and HSnO2- are __.
Determine the volume (L) of hydrogen needed (based on room temperature 25 °C and l atm) to hydrotreat 1 liter crude oil with 1.8 wt % S to a sulfur content of 0.2 wt%. The oil has a specific gravity of 0.8 (compared to water). The stoichiometry follows the following reaction: Oil-S + 2H2¬→O¡I-H2 + H2S
You dissolve 150.0 grams of a mixture of MgCl2 and KCI in water, add a solution of excess AgNO3, and precipitate all of the chloride ion as AgCI. After filtration and drying, you find that you have formed 335.0 grams of AgCl. What is the mass in grams of KCI that was in the original 150.0 grams of mixture? g 1 4 C 7 8. +/- x 100 Tap here or pull up for additional resources 3. LO
The graph below corresponds to the system below at standard conditions A = 2B 0.100 0.080 0.060 B 0.040 A 0.020 0.000 0.00 1.00 2.00 Time (min) Which statement is correct? OK1;AG >0 OK 1; AG <0 Concentration (M) 3.00 4.00 5.00
Bb Blackboard Collaborate Ultra – 1 X Bb Collaborate - Live Tutorial Ses X O Mail - Jenna Lewis - Outlook G 0.615 ml to litres - Google Search x + 8 https://au.bbcollab.com/collab/ui/session/playback Not syncing O Instagram (11) Facebook In class questions: Q97: - Use the provided equation to calculate the following: KMNO, (aq) + HCI (aq) → MnCl, (aq) + Cl, (g) + H,0 (1) + KCI (aq) а. The moles of H,0 that can be obtained from 15.0 ml of 0.250 M HCI. b. The volume of 0.150 M KMno, needed to produce 0.0143 g MnCl,. С. The volume of 2.50 M HCI needed to produce 125 ml of 0.525 M KCI. 2KMNO, (aq) + 16HCI (aq) → 2MNCI, (aq) + 5Cl, (g) + 8H,0 (1) + 2KCI (aq) 01:36:12 01:59:28 [CC 9:14 AM O Type here to search 14°C Sunny 4) ENG 9/09/2021
_engine.html?ClassID=D1746475745#3 Drie. 0.700 g of an impure FeCl3 sample are dissolved in water to create 50.0 mL of solution. It took 1.54 x 10-3 mol H2C204 to reach the equivalence point. 2FE3+ (aq) + H2C204(aq) CO2(g) + 2H*(aq) + 2FE2+(aq) -> How many moles of FeCl3 react? 1? [? ]x 10 mol FeCl3 Coefficient (green) Exponent (yellow) Enter