ChemistryPlease estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3 2. AldolaseAldolase is a 157 kD enzyme that catalyzes the following reaction:AldolaseOHHOOHD-Fructose-1,6-diphosphate2.551025501[s] (uM) Vo (uM/min)13.98.115.322.028.033.0rofalanDihydroxyacetone phosphateD-Glyceraldehyde-3-phosphateIn an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detectedby modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). Theenzyme concentration for the assay is 150 pM, and the following data is obtained:OHH₂NNH₂OH

Estimation of Km and Vmax can be done from the Michaelis-Menten equation. The values of Km and Vmax…

Answered: Please estimate the Km and Vmax and…

Please estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Chemistry

Please estimate the Km and Vmax and then calculate the kinetic constants (Km, Vmax, kcat, and kcat/Km) Please and thank you <3

2. Aldolase
Aldolase is a 157 kD enzyme that catalyzes the following reaction:
Aldolase
OH
HO
OH
D-Fructose-1,6-diphosphate
2.5
5
10
25
50
1
[s] (uM) Vo (uM/min)
1
3.9
8.1
15.3
22.0
28.0
33.0
rofalan
Dihydroxyacetone phosphate
D-Glyceraldehyde-3-phosphate
In an assay of Aldolase (100 µL volume), the production of D-Glyceraldehyde-3-phosphate (GAP) is detected
by modification with excess hydrazine to give a hydrazone that absorbs at 240 nm (624028500 M¹cm¹¹). The
enzyme concentration for the assay is 150 pM, and the following data is obtained:
OH
H₂NNH₂
OH

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