An HPLC method was developed for the separation and determination of ibuprofen in blood samples. A 3.00 mL blood sample dissolved in 70.00 mL solvent was prepared and 10.0 mL was injected into the HPLC. Standard ibuprofen solutions were also run. Calculate the ibuprofen present in the 3.00 mL blood sample The following data were obtained. Table po itoo:  Ibuprofen concentration, mg/ml 0.50  1.0  2.0  3.0   Blood sample   Peak area (Right column) 5.2 9.6 17.4 19.9 11.4

Fundamentals Of Analytical Chemistry
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Chapter16: Applications Of Neutralization Titrations
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An HPLC method was developed for the separation and determination of ibuprofen in blood samples. A 3.00 mL blood sample dissolved in 70.00 mL solvent was prepared and 10.0 mL was injected into the HPLC. Standard ibuprofen solutions were also run.
Calculate the ibuprofen present in the 3.00 mL blood sample

The following data were obtained.

Table po itoo: 
Ibuprofen concentration, mg/ml
0.50 
1.0 
2.0 
3.0  
Blood sample  

Peak area (Right column)
5.2
9.6
17.4
19.9
11.4

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