Prepare 100 μL of 10.0 mM hydrolyzed sucrose in 10.0 mM acetate pH 4.8 as a standard, in a 1.5 mL microcentrifuge tube, also incorporating 5.00 μL of 5.0 mM tris pH 7.4 within the 100 μL volume. Using a stock solution of 20.0 mM sucrose substrate in 10.0 mM acetate, calculate the volumes of hydrolyzed sucrose, acetate, and dH2O.
Prepare 100 μL of 10.0 mM hydrolyzed sucrose in 10.0 mM acetate pH 4.8 as a standard, in a 1.5 mL microcentrifuge tube, also incorporating 5.00 μL of 5.0 mM tris pH 7.4 within the 100 μL volume. Using a stock solution of 20.0 mM sucrose substrate in 10.0 mM acetate, calculate the volumes of hydrolyzed sucrose, acetate, and dH2O.
The total volume of the solution containing sucrose, acetate and tris is 100.
In this solution, the volume of tris = 5.00 .
To calculate the volume of acetate, we have to calculate the milimole of acetate present in it.
let , volume of acetate = y mL.
For, acetate, the volume present in the solution mixture, = 1.58.
Volume for 10mM hydrolysed sucrose will be 100-(1.58+5.00) = 93.42 .
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